Influence of Emdogain® Treatment onto K2O Incorporated Calcium Metaphosphate on Osteogenic Activation

Suk Young Kim; Chang Kuk You; Jae Ho Jeong; Eui Kyun Park; Shin Yoon Kim; Hong In Shin

doi:10.4028/www.scientific.net/KEM.284-286.631

Paper Titles

Bisphosphonates Incorporated on a Ca-P Biomimetic Coating Produced by a Sodium Silicate Based Methodology Stimulate Osteoblastic Activity
p.615

Solution Mediated Effect of Bioactive Glass in Poly (Lactic-Co-Glycolic Acid)- Bioactive Glass Composites on Osteogenesis of Marrow Stromal Cells
p.619

In Situ Monitoring of Chondrocyte Response to Bioactive Scaffolds Using Raman Spectroscopy
p.623

Incorporation of Human Osteoblast Cells and Osteoblast-Like Cells into Porous Hydroxyapatite Scaffolds
p.627

Influence of Emdogain^® Treatment onto K₂O Incorporated Calcium Metaphosphate on Osteogenic Activation
p.631

Evaluation of Osteoblast Viability, Alkaline Phosphatase Production and Collagen Secretion in the Presence of Hydroxyapatite Reinforced with Oxide Glasses
p.635

Biocompatibility Evaluation of Three Different Titanium-Hydroxyapatite Composites
p.639

Biomimetic Hydroxyapatite Micro-Tube Tissue Scaffold
p.643

Effects of Cyclic RGD Peptide Functionalization on the Quantitative Bone Ingrowth Process in Cellularized Biphasic Calcium Phosphate Ceramics
p.647

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Influence of Emdogain^® Treatment onto K₂O Incorporated Calcium Metaphosphate on Osteogenic Activation

Abstract:

As a part of the efforts to develop a suitable scaffold optimizing bone regeneration that has similar physical properties to bone, we modified calcium metaphosphate (CMP) ceramics with K2O and evaluated their efficiency as a scaffold for tissue engineered bone tissue regeneration. Macroporous CMP ceramics modified by incorporation of 5% K2O to improve biodegradability were prepared to have 250 and 450 µm average pore sizes, respectively. The modified CMP ceramics were cultured with mouse primary calvarial osteoblastic cells in osteogenic media for 2 weeks and these cell-CMP ceramic constructs with or without Emdogain treatment were implanted in the SCID mice subcutaneous pouches. After 1, 2, and 3 weeks, the degree of ectopic bone formation was evaluated. The modified macroporous CMP ceramic-cell constructs treated with Emdogain induced ectopic bone formation, whereas the modified CMP ceramic-cell constructs without Emdogain treatment induced no ectopic bone formation. This result suggests that the Emdogain treatment on cell-scaffold constructs for tissue engineered bone regeneration may be effective for osteogenic activation of attached cells.