The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro

Abstract:

The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10µM) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type I, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10µM orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased.

• Periodical: Key Engineering Materials (Volumes 309-311)
• Pages: 121-124
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.309-311.121
• Online since: May 2006
• Authors: Meera Q. Arumugam, D.C. Ireland, Roger A. Brooks, Neil Rushton, William Bonfield
• Keywords: Alkaline Phosphatase, Collagen Type I, Human Osteoblast Cells, Orthosilicic Acid, Osteocalcin, Reverse Transcriptase-Polymerase Chain Reaction, Silicon

References

[1] N Patel et al., J. Mat. Sci.: Mat. in Med. 13, (2002).

[2] I Gibson et al., J. Biomed. Res. 44, p.422, (1999).

[3] D M Reffitt et al., Orthosilicic acid stimulates collagen type I synthesis and osteoblastic differentiation in human osteoblast-like cells in vitro, Bone 32, pp.127-135, (2003).

DOI: 10.1016/s87563282(02)00950-x

[4] M Q Arumugam et al., Orthosilicic Acid Increases Collagen Type I mRNA Expression in Human Bone-Derived Osteoblasts In Vitro, Key Eng. Mat. 254-2, pp.869-872, (2004).

DOI: 10.4028/www.scientific.net/kem.254-256.869

[5] D E Ireland et al., Hydrocortisone increases the rate of differentiation of cultured human osteoblasts, J. Cell. Biochem. 91 (3), pp.594-601, (2004).

DOI: 10.1002/jcb.10756

[6] D E Ireland et al., Multiple changes in gene expression in chronic human Achilles tendinopathy, Matrix Biology 20, pp.159-169, (2001).

DOI: 10.1016/s0945-053x(01)00128-7

[7] K L Tucker et al., Dietary silicon and bone mineral density: The Framingham study, J. Bone Min. Res. 16: S510-S510, (2001).

[8] R Jugdaohsingh et al., Dietary silicon intake and absorption., Am. J. Clin. Nutr. 75(5): p.887893, (2002).

[9] M C Hott et al., Short-Term Effects of Organic Silicon on Trabecular Bone in Mature Ovariectomized Rats., Calc. Tiss. Int. 53(3): pp.174-179.

DOI: 10.1007/bf01321834

[10] E M Carlisle et al., Science 167 & Calc. Tiss. Int. 33, p.27, (1981).