The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro

Abstract:

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The object of this study was to investigate the effect of the concentration of orthosilicic acid (0, 0.5, 1, 5 and 10µM) on gene expression in human osteoblast cells isolated from trabecular bone. This was measured using reverse transcriptase-polymerase chain reaction (RT-PCR) to quantify messenger RNA (mRNA) levels for collagen type I, alkaline phosphatase and osteocalcin. Results showed that while collagen type I mRNA expression was increased by the addition of up to 10µM orthosilicic acid, ALP message was suppressed over time and osteocalcin levels were decreased.

Info:

Periodical:

Key Engineering Materials (Volumes 309-311)

Main Theme:

Edited by:

Takashi Nakamura, Kimihiro Yamashita and Masashi Neo

Pages:

121-124

DOI:

10.4028/www.scientific.net/KEM.309-311.121

Citation:

M. Q. Arumugam et al., "The Effect Orthosilicic Acid on Collagen Type I, Alkaline Phosphatase and Osteocalcin mRNA Expression in Human Bone-Derived Osteoblasts In Vitro ", Key Engineering Materials, Vols. 309-311, pp. 121-124, 2006

Online since:

May 2006

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Price:

$35.00

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