Adhesion, Proliferation and Morphology of Osteoblasts Cultured on Apatite Ceramics with Preferred Orientation to a-Plane
There are two crystal planes in hydroxyapatite (Ca10(PO4)6(OH)2; HAp) that is clinically applied to artificial bone or dental root. The two crystal planes, a- and c-planes, are anisotropy. We have successfully fabricated dense HAp ceramics with preferred orientation to a-plane from single-crystal apatite fibers (AF) and apatite gels (AG). We examined the cellular response, such as adhesion, proliferation and morphology, of orthoclastic MC3T3-E1 cells, to the surface of the HAp ceramics with preferred orientation to a-plane. The initial cell attachment efficiency of the HAp ceramics with a-plane was lower than that of isotropic HAp ceramics as a control. This may be due to the difference of surface potential of the examined HAp ceramics. The proliferation of cells cultured on the HAp ceramics with a-plane was almost the same as that of isotropic HAp one.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
A. Miyazaki and M. Aizawa, "Adhesion, Proliferation and Morphology of Osteoblasts Cultured on Apatite Ceramics with Preferred Orientation to a-Plane ", Key Engineering Materials, Vols. 309-311, pp. 109-112, 2006