Stability of Cultured Osteoblasts on Calcium Phosphate Ceramics

Akira Oshima; Mika Tadokoro; Motohiro Hirose; Yoshinori Takakura; Hajime Ohgushi

doi:10.4028/www.scientific.net/KEM.309-311.93

Paper Titles

Mesoporous Structure and Protein Adsorption Property of c-Axis Oriented Apatite Aggregates
p.77

Protein Adsorption Ability of Porous Hydroxyapatite Microparticle with Metal Ions
p.81

Effect of Mg on Surface Roughness and Protein Content of Protein-Apatite Composite Layers
p.85

Effects of Through-holes' Diameter on Initial Cell Attachment and Cell Proliferation
p.89

Stability of Cultured Osteoblasts on Calcium Phosphate Ceramics
p.93

Novel Calcium Phosphate Ceramics: The Remarkable Promoting Action on the Differentiation of the Normal Human Osteoblasts
p.97

Sintering Effect on Mechanical Properties of Composites of Hydroxyapatite Lanthanum Oxide (HA-La₂O₃)
p.101

Osteoblast Proliferation and Morphology Analysis on Laser Modified Hydroxyapatite Surfaces: Preliminary Results
p.105

Adhesion, Proliferation and Morphology of Osteoblasts Cultured on Apatite Ceramics with Preferred Orientation to a-Plane
p.109

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Stability of Cultured Osteoblasts on Calcium Phosphate Ceramics

Abstract:

Cultured osteoblasts with mineralized matrix (regenerative cultured bone; RCB) have been used for patients having osseous defects. The RCB can be fabricated on various ceramic substrata using patient’s mesenchymal stem cells (MSCs) at our cell processing center (CPC). Since we have to transport the RCB for hospitals outside of our town, the RCB should maintain the cell viability for a long time. To determine a suitable condition for transportation of the RCB, stability of the RCB was analyzed by biochemical assays. Even outside CO2 incubator, the RCB kept high level of viability until 24 hours at 25°C and also showed low level of cytotoxicity for 24 hours at 37°C and 25°C. On the other hand, the RCB incubated for 24 hours at 4°C outside CO2 incubator resulted in extremely low level of viability with obvious cytotoxicity. These data indicated that stability of the RCB can be maintained for 24 hours at 37°C and 25°C, but not at 4°C. Therefore, the RCB derived from patient’s MSCs can be transported and utilized for the patients at hospitals far away from the CPC.