Effect of Mg on Surface Roughness and Protein Content of Protein-Apatite Composite Layers

Yuusuke Ishikawa; Nao Kondo; Yu Sogo; Atsuo Ito; Eiji Uchimura; Ayako Oyane; Tadao Ohno; Atsushi Yamazaki; Takashi Ushida; Katsuko S. Furukawa

doi:10.4028/www.scientific.net/KEM.309-311.85

Paper Titles

Progress of Structural Elucidation of Amorphous Calcium Phosphate(ACP)and Hydroxyapatite (HAp): Disorder and Surfaces as Seen by Solid State NMR
p.69

Bovine Serum Albumin Adsorption on Hydroxyapatite and Biphasic Calcium Phosphate and the Correlation with Zeta Potentials and Wettability
p.73

Mesoporous Structure and Protein Adsorption Property of c-Axis Oriented Apatite Aggregates
p.77

Protein Adsorption Ability of Porous Hydroxyapatite Microparticle with Metal Ions
p.81

Effect of Mg on Surface Roughness and Protein Content of Protein-Apatite Composite Layers
p.85

Effects of Through-holes' Diameter on Initial Cell Attachment and Cell Proliferation
p.89

Stability of Cultured Osteoblasts on Calcium Phosphate Ceramics
p.93

Novel Calcium Phosphate Ceramics: The Remarkable Promoting Action on the Differentiation of the Normal Human Osteoblasts
p.97

Sintering Effect on Mechanical Properties of Composites of Hydroxyapatite Lanthanum Oxide (HA-La₂O₃)
p.101

HomeKey Engineering MaterialsKey Engineering Materials Vols. 309-311Effect of Mg on Surface Roughness and Protein...

Effect of Mg on Surface Roughness and Protein Content of Protein-Apatite Composite Layers

Abstract:

Fibronectin (FN)-apatite composite layers were formed on a hydroxyapatite (HAP) ceramic using supersaturated calcium phosphate (CP) solutions. The surface roughness of the composite layer could be controllable by changing the magnesium (Mg) concentration in the CP solution. The higher the Mg concentration, the smaller the apatite crystals in the layer were. The thickness of the layer formed in the CP solution containing 1.5 mM Mg decreased to 68% that formed in the Mg-free CP solution. The amount of FN immobilized in the layer was almost constant regardless of Mg concentration in the CP solution in the range from 0 to 1.5 mM. The results of the present study revealed that a thin FN-apatite composite layer with a smooth surface and a high protein content is formed in a CP solution at a Mg concentration of 1.5 mM.