Osteoblast Proliferation and Morphology Analysis on Laser Modified Hydroxyapatite Surfaces: Preliminary Results

Abstract:

Biocompatibility has long been associated with surface microtopography, microtexture and microchemistry. The surface topography ultimately affects the nature and the strength of the interactions that occur at biomaterial-biological environment (cell adhesion, mobility, spreading and proliferation). Thus, it is necessary to produce and work with controlled microtopographical surfaces that present reproducible microdomains of a dimension similar to that of the biological elements of interest (for instance, cells). [1] There are a number of substrates that already have been studied (such as silicone, polystyrene, poly-L-lactic acid and titanium coated polystyrene) in terms of surface topography. [2] However, few studies are related to hydroxyapatite substrates. As it is well established, hydroxyapatite is a well known ceramic that is extremely used in medical applications, namely implants and coatings. In this work, the surface topography of dense hydroxyapatite substrates was altered by using KFr excimer laser. Excimer lasers produce high-intensity, pulsed ultraviolet radiation and are especially well suited for materials processing due to their large beam cross-section area, which permits using mask projection technologies to process relatively large areas in a single step.[3]

• Periodical: Key Engineering Materials (Volumes 309-311)
• Pages: 105-108
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.309-311.105
• Online since: May 2006
• Authors: S. Teixeira, A.C. Queiroz, F.J. Monteiro, M.P. Ferraz, Rui Vilar, S. Eugénio
• Keywords: Cell Adhesion, Excimer Laser, Hydroxyapatite (HAP), Surface Modification

References

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[2] Walboomers, X. F. et al Contact guidance of rat fibroblasts on various implant materials, Journal of Biomedical Materials Research, 47 (1999) 204-212.

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[3] Queiroz A .C. et al, Laser surface modifications of hydroxyapatite and glass-reinforced hydroxyapatite, Biomaterials 25 (2004) 4607-4614.

DOI: 10.1016/j.biomaterials.2003.11.054

[4] Ahmad M, Mc Carthy MB, Gronowicz G. An in vitro model for mineralization of human osteoblasts-like cells on implant materials. Biomaterials, 1999; 20: 211-20.

DOI: 10.1016/s0142-9612(98)00152-5