Controlled Release of rhBMP-2 Loaded Poly(DL-lactic-co-glycolic Acid)/ Calcium Phosphate Cement Composites In Vivo

Abstract:

The release kinetics of recombinant human bone morphogenetic protein-2 (rhBMP-2) loaded poly(DL-lactic-co-glycolic acid)/calcium phosphate cement (PLGA/Ca-P cement) composites were studied in vivo. RhBMP-2 was radiolabeled with 131I and entrapped within PLGA microparticles or adsorbed onto the microparticle surface. PLGA microparticles were prepared of high molecular weight (HMW) PLGA (weight average molecular weight [Mw] 49,100 ± 1,700) or low molecular weight (LMW) PLGA (Mw 5,900 ± 300) and used for preparation of 30:70 wt% PLGA/Ca-P cement composite discs. Release of 131I-rhBMP-2 loaded composites was assessed by scintigraphic imaging according to a 22 two-level full factorial design in the rat ectopic model during four weeks. In vivo release kinetics varied among formulations. All formulations showed slow release without initial burst, and displayed a linear release from 3 to 28 days. Release of LMW entrapped rhBMP-2 composites (1.7 ± 0.3%/day) was significantly faster than release from other formulations (p < 0.01). After 28 days, retention within the composites was 65 ± 5%, 75 ± 4%, 50 ± 4% and 70 ± 6% of the initial rhBMP-2 for HMW entrapped, HMW adsorbed, LMW entrapped and LMW adsorbed rhBMP-2 composites, respectively. Release from the composite was probably slowed down by an interaction of rhBMP-2 and Ca-P cement after rhBMP-2 release from PLGA microparticles. We conclude that PLGA/Ca-P cement composites can be considered as sustained slow release vehicles and that the release and retention of rhBMP-2 can be modified according to the desired profile to a limited extent.

• Periodical: Key Engineering Materials (Volumes 309-311)
• Pages: 973-976
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.309-311.973
• Online since: May 2006
• Authors: P.Q. Ruhé, O.C. Boerman, F.G.M. Russel, P.H.M. Spauwen, Antonious G. Mikos, John A. Jansen
• Keywords: Bone Engineering, Calcium Phosphate, PLGA Microparticles, Release, rhBMP-2

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