Increase in the Biocompatibility of the Neutralized Chitosan Dermal Scaffold by Reconstruction of Wound Healing Microenvironment: In Vitro Study

Abstract:

The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately five folds by mixing or coating of atelomeric type I collagen. In order to recreate wound-healing microenvironment within the NCS for the better wound healing effect, various concentrations of bFGF and fibronectin (FN) were supplied in the secondary freeze-dry process of the scaffold. NCS+ bFGF and NCS+FN improved the cell binding capacity by four folds and three folds respectively. Therefore supplementation of collagen, b-FGF and/or fibronectin in the NCS can improve the biocompatibility of the chitosanbased scaffold which itself revealed poor cell binding capacity.

• Periodical: Key Engineering Materials (Volumes 342-343)
• Pages: 185-188
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.342-343.185
• Online since: July 2007
• Authors: Yong Ha Youn, Chun Ho Kim, Young Ju Choi, Yong Jae Gin, Young Sook Son
• Keywords: bFGF, Bioartificial Skin, Chitosan (CS), Collagen, Dermal Scaffold, Fibronectin

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