Increase in the Biocompatibility of the Neutralized Chitosan Dermal Scaffold by Reconstruction of Wound Healing Microenvironment: In Vitro Study
The porous neutralized chitosan scaffold (NCS) was prepared by freeze-dry method. Its poor cell binding capacity was improved approximately five folds by mixing or coating of atelomeric type I collagen. In order to recreate wound-healing microenvironment within the NCS for the better wound healing effect, various concentrations of bFGF and fibronectin (FN) were supplied in the secondary freeze-dry process of the scaffold. NCS+ bFGF and NCS+FN improved the cell binding capacity by four folds and three folds respectively. Therefore supplementation of collagen, b-FGF and/or fibronectin in the NCS can improve the biocompatibility of the chitosanbased scaffold which itself revealed poor cell binding capacity.
Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon
Y. H. Youn et al., "Increase in the Biocompatibility of the Neutralized Chitosan Dermal Scaffold by Reconstruction of Wound Healing Microenvironment: In Vitro Study", Key Engineering Materials, Vols. 342-343, pp. 185-188, 2007