Investigation of Pore Size Effect on Cell Compatibility Using Pore Size Gradient Chitosan Scaffold

Sung Mook Lim; Se Heang Oh; Il Kyu Park; Jin Ho Lee

doi:10.4028/www.scientific.net/KEM.342-343.285

Paper Titles

Evaluation of Semi-Interpenetrating Polymer Networks Composed of Chitosan and Poloxamer for Artificial Skin
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Effects of Mechanical Stress on the In Vitro Degradation of Porous Composite Scaffold for Bone Tissue Engineering
p.273

Bone Regeneration Materials
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Muscle-Derived Stem Cell/Polymer Hybrid Mixture for the Treatment of Urinary Incontinence
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Investigation of Pore Size Effect on Cell Compatibility Using Pore Size Gradient Chitosan Scaffold
p.285

Fabrication and Characterization of Hydrophilized Polydioxanone Scaffolds for Tissue Engineering Applications
p.289

Characterization of Hydrophilized PCL Electrospun Sheet as an Efective Guided Bone Regeneration Membrane
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Evaluation of RGD-Grafted Porous PLLA Scaffold for Chondrogenic Differentiation of Stem Cells
p.297

Thermosensitive and Cell-Adhesive Pluronic Hydrogels for Human Adipose-Derived Stem Cells
p.301

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Investigation of Pore Size Effect on Cell Compatibility Using Pore Size Gradient Chitosan Scaffold

Abstract:

Chitosan cylindrical scaffolds with gradually increasing pore size along the longitudinal direction were fabricated by a novel centrifugation method to investigate pore size effect on cell interactions. The scaffold was fabricated by the centrifugation of a cylindrical mold containing fibril-like chitosans. The pore size ranges of the scaffold could be controlled by adjusting the centrifugal speed: the scaffold with gradually increasing pore size (from ~80 #m to ~400 #m) and porosity (from ~82 % to ~93 %) along the cylindrical axis was obtained by applying the centrifugal speed, 3,000 rpm. The scaffold sections were examined for their in vitro cell interactions using different kinds of cells (fibroblasts, chondrocytes, and osteoblasts) in terms of scaffold pore sizes. It was observed that different kinds of cells were shown to have different pore size ranges in the scaffold for effective cell growth. The chitosan scaffold section with ~400 #m pore size showed better cell growth for chondrocytes and osteoblasts, while the scaffold section with ~190 #m pore size was better for fibroblast growth. The pore size gradient scaffolds fabricated by the centrifugation method can be a good tool for the systematic studies of the interactions between cells or tissues and scaffolds with different pore size.