Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype

Hyun Jung Lee; Keun Hong Park; So Ra Park; Byoung Hyun Min

doi:10.4028/www.scientific.net/KEM.342-343.329

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Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 342-343Chitosan/Heparin Polyelectrolyte Complex...

Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype

Abstract:

Acknowledging the supportive influence of matrix molecules on the chondrocytic phenotype, we combined heparin and chitosan to develop a novel biomaterial, supporting chondrogenesis. Chitosan had been shown as a promising structural material for a number of tissue engineering applications. Similar to heparin, one of the glycosamino-glycans (GAGs) had been known to exert various influences on the biological activities. In this study we evaluated the potential of heparin/chitosan polyelectrolyte complex materials for controlling the proliferation and differentiation of chondrocytes. The heparin/chitosan polyelectrolyte complex was coated over the polyethyleneimine (PEI) layer precoated on the commercial polystyrene dish. When examined by using human chondrocyte cell line (C28/I2), the heparin/chitosan surfaces supported well not only the cell proliferation but also the chondrocytic functions, such as expression of collagen type II. These results indicated that the heparin/chitosan complex could be used to modulate the activity of chondrocytes in vitro.

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Periodical:

Key Engineering Materials (Volumes 342-343)

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329-332

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.342-343.329

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Online since:

July 2007

Authors:

Hyun Jung Lee, Keun Hong Park, So Ra Park, Byoung Hyun Min

Keywords:

Chitosan (CS), Heparin, Nanoparticle Culture System, Polyethyleneimine

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