Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype

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Acknowledging the supportive influence of matrix molecules on the chondrocytic phenotype, we combined heparin and chitosan to develop a novel biomaterial, supporting chondrogenesis. Chitosan had been shown as a promising structural material for a number of tissue engineering applications. Similar to heparin, one of the glycosamino-glycans (GAGs) had been known to exert various influences on the biological activities. In this study we evaluated the potential of heparin/chitosan polyelectrolyte complex materials for controlling the proliferation and differentiation of chondrocytes. The heparin/chitosan polyelectrolyte complex was coated over the polyethyleneimine (PEI) layer precoated on the commercial polystyrene dish. When examined by using human chondrocyte cell line (C28/I2), the heparin/chitosan surfaces supported well not only the cell proliferation but also the chondrocytic functions, such as expression of collagen type II. These results indicated that the heparin/chitosan complex could be used to modulate the activity of chondrocytes in vitro.

Info:

Periodical:

Key Engineering Materials (Volumes 342-343)

Edited by:

Young-Ha Kim, Chong-Su Cho, Inn-Kyu Kang, Suk Young Kim and Oh Hyeong Kwon

Pages:

329-332

DOI:

10.4028/www.scientific.net/KEM.342-343.329

Citation:

H. J. Lee et al., "Chitosan/Heparin Polyelectrolyte Complex Nanoparticles (100~200nm) Covalently Bonded with PEI for Enhancement of Chondrogenic Phenotype ", Key Engineering Materials, Vols. 342-343, pp. 329-332, 2007

Online since:

July 2007

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$38.00

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DOI: 10.1002/jcp.10397

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