Optimization of Scaffold for a Successful Hydrogel-Seeding Method for Vascular Tissue Engineering

Abstract:

Porosity and pore size are needed for successful cell seeding and proliferation into porous scaffolds. This study was focused on a hydrogel-seeding method to improve cell adhesion and proliferation in tubular porous scaffolds for vascular grafts application. Tubular scaffolds were fabricated from a biodegradable elastic polymer, poly(L-lactide-co-ε-caprolactone) (PLCL) (50:50, Mn 1.58×105), by an extrusion-particulate leaching method. Vascular smooth muscle cells (VSMCs) were dispersed in collagen hydrogel and then seeded into the tubular PLCL scaffolds having various pore sizes, 50-100 μm, 100-200 μm, and 300-500 μm, respectively. As a result, the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds. Especially, the cell proliferation efficiency was improved by using the hydrogel-seeding method as compared with by using a previously established method. In summary, this study demonstrates that the efficiency of cell adhesion and proliferation was dependent on the pore size of the scaffolds in the hydrogel-seeding method.

• Periodical: Key Engineering Materials (Volumes 342-343)
• Pages: 333-336
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.342-343.333
• Online since: July 2007
• Authors: In Su Park, Sang Heon Kim, Jeong Woo Han, Young Gun Ko, Eun Na Chung, Soo Hyun Kim
• Keywords: Collagen, Hydrogel, Pore Size, Smooth Muscle Cells, Vascular Tissue Engineering

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