Cellular Responses of MC3T3-E1 Mouse Osteoblast-Like Cells to Rough Surfaced Substrates

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MC3T3-E1 mouse osteoblast-like cells were seeded at high cell density to form confluent monolayer on rough surfaced culture substrates. Osteoblastic gene and protein expressions and matrix mineralization were investigated to clarify the effect of surface roughness on differentiation of MC3T3-E1 cells.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

1071-1074

Citation:

T. Saito et al., "Cellular Responses of MC3T3-E1 Mouse Osteoblast-Like Cells to Rough Surfaced Substrates", Key Engineering Materials, Vols. 361-363, pp. 1071-1074, 2008

Online since:

November 2007

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$38.00

[1] T. Saito, H. Hayashi, K. Uoe, T. Kizuki, K. Teraoka, K. Kato and Y. Yokogawa: Key Eng. Mater. Vol. 284-286 (2005), p.573.

[2] A. Ehara, K. Ogata, S. Imazato, S. Ebisu, T. Nakano and Y. Umakoshi: Biomaterials Vol. 24 (2003), p.831.

[3] S. Reppe, L. Stilgren, O.K. Olstad, K. Brixen, L.S. Nissen-Meyer, K.M. Gautvik and B. Abrahamsen: Bone Vol. 39 (2006), p.189.

DOI: https://doi.org/10.1016/j.bone.2005.12.020

[4] M.M. Bradford: Anal. Biochem. Vol. 72 (1976), p.248.

0.

5.

[1] 0.

[1] 5.

[2] 0.

[2] 5.

[3] 0 ALP BGP Runx2 Col1A1 OPN.

[3] 77.

[1] 99.

98.

48.

04 Ra (µµµµm) Expression rate (-) 0 10 20 30 40 50.

[3] 77 1. 99 0. 98 0. 48 0. 04 Ra (µµµµm) BGP(ng/hr/mg-protein) 0 200 400 600 800 1000.

[3] 77 1. 99 0. 98 0. 48 0. 04 Ra (µµµµm) ALP (U/mg-protein).

[3] 77 1. 99 0. 98 0. 48 0. 04 Ra (µµµµm) AR-S (mg/cm 2) 0 1 2 3 4 5.

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