Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture

Abstract:

Article Preview

The bone-like self-organized hydroxyapatite/collagen (HAp/Col) nanocomposite sponge was prepared from HAp/Col nanocomposite fibers. We analyzed osteogenic activity of human osteoblastic MG63 cells in the HAp/Col sponge under pressure/perfusion culture. Collagen (Col) sponge was used as a control. The MG63 cells were attached well and showed good proliferation in the HAp/Col sponge. The total DNA content in the HAp/Col sponge was approximately 1.8 times greater than that in the Col sponge at 21 days. The MG63 cells showed good osteogenic gene expression in the HAp/Col sponge by reverse transcription-polymerase chain reaction analysis. These result suggested that HAp/Col sponge can be useful for bone tissue engineering scaffold materials.

Info:

Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

1079-1082

Citation:

T. Yoshida et al., "Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture", Key Engineering Materials, Vols. 361-363, pp. 1079-1082, 2008

Online since:

November 2007

Export:

Price:

$38.00

[1] Rea. SM, Best SM, Bonfield W: J Mater Sci Mater Med Vol. 15 (9): pp.997-1005 (2004).

[2] D.A. Wahl and J.T. Czernuszka: European Cells and Materials. Vol. 11 p.43 (2006).

[3] M. Kikuchi, S. Itoh, S. Ichinose, K. Shinomiya, and J. Tanaka: Biomaterials Vol. 22 p.1705 (2001).

[4] M. Kikuchi, T. Ikoma, D. syoji, H.N. Matsumoto. Y. Koyama, S. Itoh, K. Takakuda, S, K. Shinomiya, and J. Tanaka: Key-Eng. Mater. Vol. 254-256 pp.561-564 (2004).

DOI: https://doi.org/10.4028/www.scientific.net/kem.254-256.561

[5] J. Glowacki, S. Mizuno, JS Greenberger: Cell Transplant. Vol. 7 (3) pp.319-326 (1998).

[6] M. Kikuchi, K. Kikuchi, K. Johnson, J. Glowacki: J Oromaxillofacial Biomech Vol. 10 (1) pp.57-60 (2004).

[7] T. Yoshida, M. Kikuchi, Y. Koyama and K. Takakuda: Key-Eng. Mater. Vol. 330-332 pp.317-320 (2007).

Fetching data from Crossref.
This may take some time to load.