Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture

Teruaki Yoshida; Ikko Mizuno; Masanori Kikuchi; Yoshihisa Koyama; Kazuo Takakuda

doi:10.4028/www.scientific.net/KEM.361-363.1079

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 361-363Changes in Osteogenic Activity of MG63 Cells on...

Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture

Abstract:

The bone-like self-organized hydroxyapatite/collagen (HAp/Col) nanocomposite sponge was prepared from HAp/Col nanocomposite fibers. We analyzed osteogenic activity of human osteoblastic MG63 cells in the HAp/Col sponge under pressure/perfusion culture. Collagen (Col) sponge was used as a control. The MG63 cells were attached well and showed good proliferation in the HAp/Col sponge. The total DNA content in the HAp/Col sponge was approximately 1.8 times greater than that in the Col sponge at 21 days. The MG63 cells showed good osteogenic gene expression in the HAp/Col sponge by reverse transcription-polymerase chain reaction analysis. These result suggested that HAp/Col sponge can be useful for bone tissue engineering scaffold materials.

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Key Engineering Materials (Volumes 361-363)

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1079-1082

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1079

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Online since:

November 2007

Authors:

Teruaki Yoshida, Ikko Mizuno, Masanori Kikuchi, Yoshihisa Koyama, Kazuo Takakuda

Keywords:

Hydroxyapatite Nanocomposite Sponge, Osteogenic Activity, Perfusion Culture, Pressure Culture

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