Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray

S.H. Park; J.M. Park; H.S. Lee; K.S. Min; A.R. Pae; N.R. Kang

doi:10.4028/www.scientific.net/KEM.361-363.1091

Paper Titles

Microenvironment of Apatite-Fiber Scaffold Affects Cell Proliferation and Resulting Cell Differentiation
p.1075

Changes in Osteogenic Activity of MG63 Cells on Hydroxyapatite/Collagen Nanocomposite Sponge under Pressure/Perfusion Culture
p.1079

Comparison of the Biological Behavior of Wollastonite Bioceramics Prepared from Synthetic and Natural Precursors
p.1083

Biological and Biomechanical Behavior of Titanium Implants with Fluoridated Hydroxyapatite Coatings
p.1087

Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray
p.1091

Comparison of Gene-Expression of MC3T3-E1 Osteoblastic Cell Cultured on Machined Titanium Surface and Resorbable Blast Material Titanium Surface
p.1095

Proliferation of Osteoblast-Like Cells on Zirconia/Alumina Nanocomposite
p.1099

The Experimental Study of Culture of Osteoblast-Like Cells (MG-63) on Nanometer Magnetic Composite Substrate In Vitro
p.1103

Rat Bone Marrow Cell Responses on the Surface of Hydroxyapatite with Different Topography
p.1107

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Gene-Expression of MC3T3-E1 Osteoblast Cells Cultured on Anodized Titanium Surface and Machined Titanium Surface by Using cDNA Microarray

Abstract:

One of the techniques for modifying Ti implant surface is an anodization. This method is an electrochemical one that forms rough, thick, and porous oxide films. To compare the gene expression from the cells cultured on the different implant surface, cDNA microarray have been performed. MC3T3-E1 cells were cultured on titanium disks. The control groups were cultured on the machined surfaces(MS). The experimental groups cultured on the anodized titanium surfaces(AS). Microarray assay 48 hours after culturing the cells on the machined surface and anodized surface revealed that osteoinductive molecules appeared more prominent on the anodized surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than anodized surface, which will affect the phenotype of the plated cells depending on the surface morphology.