Characterization of Bone Cell Behaviors on Collagen Grafted Hydroxyapatite Surfaces

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In order to enhance bone cell adhesion on hydroxyapatite (HA), collagen was used as a surface-grafting material on HA substrates because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent on HA disk surfaces. MC3T3-E1 osteoblasts were seeded on the collagen-grafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 4 hrs to evaluate the cell adhesion on the HA samples. The osteoblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen

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Periodical:

Key Engineering Materials (Volumes 361-363)

Main Theme:

Edited by:

Guy Daculsi and Pierre Layrolle

Pages:

1143-1146

Citation:

S. Chun et al., "Characterization of Bone Cell Behaviors on Collagen Grafted Hydroxyapatite Surfaces", Key Engineering Materials, Vols. 361-363, pp. 1143-1146, 2008

Online since:

November 2007

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$38.00

[1] S. Weiner, H.D. Wagner, Annu. Rev. Mater. Sci. 28 (1998) pp.271-298.

[2] S. Shi, M. Kirk, A.J. Kahn, J. Bone Miner. Res. 11 (1998) pp.3938-3944.

[3] M. Bigerelle, K. Anselme, J. Biomed. Res. (2005) pp.530-540.

[4] K-H. Jung, S. Kim, etc, Key Engineering Materials 330-332 (2007). HA Collagen-coated HA 1 2 3 4 5 6.

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