Preparation of the Hydroxyapatite/Collagen Three-Dimensional Scaffold from its Membrane
Effect of osteogenic activities of MG63 on the HAp/Col membrane was examined at day 10 and 14 by reverse-transcript and real-time polymerase chain reactions. Osteogenic activities of MG63 were upregulated by culture them on the HAp/Col membrane in comparison to those on tissue culture polystyrene. The novel three-dimensional HAp/Col scaffold was prepared from the HAp/Col wavy membrane. The cylindrical HAp/Col scaffold was successfully prepared and indicated at least 2.5-times higher compressive strength and Young's modulus compared to the previous HAp/Col composites. The novel scaffold could be useful for regenerative medicine.
Guy Daculsi and Pierre Layrolle
M. Kikuchi "Preparation of the Hydroxyapatite/Collagen Three-Dimensional Scaffold from its Membrane", Key Engineering Materials, Vols. 361-363, pp. 431-434, 2008