Novel Design of Confocal Fluorescence Detecting System for Capillary Array and Microchip DNA Fragment Analysis

Hua Zheng; Li Qiang Wang; Yan Shi; Jie Wang; Zu Kang Lu

doi:10.4028/www.scientific.net/KEM.364-366.1147

Paper Titles

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Modulation Transfer Function for a Multichannel Laser Induced Fluorescence Analyzer and its Numerical Calculation
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Correlation Study between a New Interferometric Asphere Metrology System and Fizeau Interferometer
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Fast Photoacoustic System Based on an Acoustic Lens and the Peak Detection Technology
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Machinability in Precision Grinding of Aspheric Optical Lens with Diamond Wheel
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HomeKey Engineering MaterialsKey Engineering Materials Vols. 364-366Novel Design of Confocal Fluorescence Detecting...

Novel Design of Confocal Fluorescence Detecting System for Capillary Array and Microchip DNA Fragment Analysis

Abstract:

A novel multi-channel DNA fragment analysis system is presented，which is based on single PMT confocal fluorescence detection and optical scanning adopting an f-theta lens. Capillary electrophoresis experiments were executed in the system for DNA marker pBR322/Hae III. Signal was processed with wavelet denoising. The system’s limit of detection (dsDNA with the probe of Thiazole Orange, TO) was evaluated to be 1.1841×10-11mol/L. Its working noise is much lower compared with that based on mechanical scanning one, and the stability and detecting sensitivity is high. The system was expected to be applied to both capillary array and microchip electrophoresis detection based on laser-induced fluorescence.

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Periodical:

Key Engineering Materials (Volumes 364-366)

Pages:

1147-1150

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.364-366.1147

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Online since:

December 2007

Authors:

Hua Zheng, Li Qiang Wang, Yan Shi, Jie Wang, Zu Kang Lu

Keywords:

Capillary Array, Confocal, DNA Fragment Analysis, Fluorescence, Microchips

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