Effect of Saline Precipitation on the Production of Collagen-Hydroxyapatite (1:1 wt%) Composite
Composites based on hydroxyapatite (HA) and collagen are being used for bone engineering applications. Synthetic composites were produced by precipitating hydroxyapatite in a reaction medium containing collagen fibers. The effect of saline treatment during the procedure of fibrils extraction on the composite morphology, phases presented and in vitro stability was evaluated. The FTIR analysis showed collagen bands well preserved. By Rietveld analysis, additional peaks of sodium chloride were identified on sample made from collagen submitted to saline precipitation. Besides that, the mineral formed phase is a carbonated apatite. This step during fibrils extraction results in larger areas of collagen uncoated and higher stability in culture medium. Despite of this, a cross-linking agent will be necessary to maintain the composite in culture for longer times.
Marcelo Prado and Cecília Zavaglia
D. M. Campos et al., "Effect of Saline Precipitation on the Production of Collagen-Hydroxyapatite (1:1 wt%) Composite", Key Engineering Materials, Vols. 396-398, pp. 425-428, 2009