Fibroin-Based Material from Natural Silk Can Be Associated with Alginate and Mesenchymal Progenitor Cells

Leandra S. Baptista; Carolina S.G. Pedrosa; Karina R. da Silva; Ronaldo J.F.C do Amaral; Michele C.L. Kochem; Hélio Menezes; Aris Sterodimas; José de Faria; Marcio R.R. de Oliveira; Radovan Borojevic

doi:10.4028/www.scientific.net/KEM.396-398.437

Paper Titles

Development of Gelatin/HA Membranes
p.421

Effect of Saline Precipitation on the Production of Collagen-Hydroxyapatite (1:1 wt%) Composite
p.425

Effect of Storage Conditions on Biaxial Flexure Strengths of MTA
p.429

Fabrication of Bioactive Organic Polymer by Using Apatite Nuclei-Contained Inorganic Binder
p.433

Fibroin-Based Material from Natural Silk Can Be Associated with Alginate and Mesenchymal Progenitor Cells
p.437

Hydroxyapatite-Sheet Microstructure of Shinbone
p.441

Improvement of Bioactivity of Collagen/Alginate Scaffolds by Hydroxyapatite for Tissue Engineering Cartilage
p.445

In Vitro Osteoclast Differentiation of Bone Marrow Cells on Hydroxyapatite/Collagen Self-Organized Bone-Like Nanocomposite Disk
p.449

Investigation on Screwy Microstructure of Solid-Trough Shell
p.453

HomeKey Engineering MaterialsKey Engineering Materials Vols. 396-398Fibroin-Based Material from Natural Silk Can Be...

Fibroin-Based Material from Natural Silk Can Be Associated with Alginate and Mesenchymal Progenitor Cells

Abstract:

Silks are naturally occurring polymers and fibroin, its filament core protein, has been shown to support stem cell differentiation in vitro, and promote tissue repair in vivo. The aim of this study is to develop a biomaterial based on silk-fibroin fibers that can be associated with mesenchymal progenitor cells from human perichondrium in vitro, in order to promote auricular reconstruction in vivo. Silk-fibroin concentrate was dissolved with formic acid solution and freeze-dried in auricular moulds. Fibroin-based material was characterized by scanning electron microscopy and by cytotoxic assays. Perichondrium mesenchymal progenitor cells were characterized by flow cytometry. They expressed the standard mesenchymal stem cell markers, and were able to differentiate into several mesenchymal lineages in vitro. This fibroin-based material is a three-dimensional fibrillar scaffold, non-woven and biocompatible, which was also well integrated with alginate and mesenchymal cells.

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Periodical:

Key Engineering Materials (Volumes 396-398)

Pages:

437-440

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.396-398.437

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Online since:

October 2008

Authors:

Leandra S. Baptista, Carolina S.G. Pedrosa, Karina R. da Silva, Ronaldo J.F.C do Amaral, Michele C.L. Kochem, Hélio Menezes, Aris Sterodimas, José de Faria, Marcio R.R. de Oliveira, Radovan Borojevic

Keywords:

Fibroin-Based Material, Perichondrium Mesenchymal Progenitor Cells, Tissue Engineering

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