In Vitro Osteoclast Differentiation of Bone Marrow Cells on Hydroxyapatite/Collagen Self-Organized Bone-Like Nanocomposite Disk

Abstract:

Osteoclast differentiation of bone marrow cells on hydroxyapatite/collagen self-organized bone-like nanocomposite (HAp/Col) disk in vitro was evaluated by coculture of mouse bone marrow cells with mouse osteoblasts with or without addition of osteoclastic inducers, 1,25-Dihydroxyvitamin D3 and 1 µM prostaglandin E2. Dentine slice and tissue culture polystyrene were used as controls. Good osteoclastic differentiation at day 7 were observed among the bone marrow cells cultured on the HAp /Col disk and controls with osteoclastic inducers. On the contrary, osteoclastic differentiation was observed only for marrow cells cultured on the HAp/Col disk. Nano- and micro- structures as well as chemical and mechanical properties have a potential to control cell differentiation.

• Periodical: Key Engineering Materials (Volumes 396-398)
• Pages: 449-452
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.396-398.449
• Online since: October 2008
• Authors: Masanori Kikuchi, Atsushi Irie
• Keywords: Collagen, Differentiation, Hydroxyapatite (HAP), Nanocomposite, Osteoclast

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