This paper carries through pure shearing fine-blanking processing experiment with negative clearance for the AISI-1020、AISI-1045 steel and the relations curves of the blanking force and the blanking stroke is obtained. The micro hardness of the sheared surface for the blanked work pieces in breadthways and lengthways direction is measured by Vickers-micro hardness instrument (MH-6), thereby the relations curves of punch stroke and the micro-hardness of the blanking work pieces are gained. The relation between work hardening degree and blanking clearance for the negative blanking is obtained too. This paper focuses on formation mechanism of work hardening for the negative blanking and analyzes the work hardening capability of blanked work piece. The work hardening degree is compared to the materials which possess different hardening index. The research shows that the work hardening degree of the blanking work pieces is strengthened evidently by the fine-blanking with negative clearance and the hardening layer of blanking fracture could enhance structure intensity and wear resistance of blanking work piece evidently. Therefore, it could enhance fatigue strength exceedingly and extend the working life of work piece greatly.