To establish clinical bone assessment for osteoporosis, it is necessary to evaluate not only bone density but also trabecular bone microstructure and mechanical properties of bone. Therefore relationship between the micro-structural parameters and the mechanical properties of the cancellous bone of bovine distal femur was investigated. Compression test was carried out using universal testing machine to measure Young’s modulus and the ultimate strength. X-ray CT was used to obtain 3D image of specimens. Bone trabecular orientation was obtained from fabric ellipse by the MIL (Mean Intercept Length) analysis. Young’s modulus and ultimate strength had a high correlation with bone density respectively; furthermore ultimate strength had a high correlation with Young’s modulus.