Study of the Microfluidic-Immunoassay Chip used to Capture Cancer Cells

Mei Hang Wei; Yi Xu; Dong Shun Li; Zhen Zhou

doi:10.4028/www.scientific.net/KEM.483.316

Paper Titles

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Numerical Simulation of Electromagnetic Actuator for Impedance Pumping
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Study on Ultrasonic Fusion Bonding for Polymer Microfluidic Chips
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Study of the Microfluidic-Immunoassay Chip used to Capture Cancer Cells
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Simulation and Experimental Study of a Porous Electroosmotic Pump
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Experimental Investigations of Anode Structure on the Performance of Air-Breathing µDMFC
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The Competition of the Retraction and Capillary Forces during the DNA Molecules Moving within Channels
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HomeKey Engineering MaterialsKey Engineering Materials Vol. 483Study of the Microfluidic-Immunoassay Chip used to...

Study of the Microfluidic-Immunoassay Chip used to Capture Cancer Cells

Abstract:

A microfluidic immunoassay chip that can selectively and specifically capture cancer cells was demonstrated. To realise the functionalization of microfluidic chip, using EDC crosslinking method, c-Met (hepatocyte growth factor receptor) was covalently linked to the channel surface of chip which was processed by means of MEMS technology. Then the functionalized immunoassay chip could be employed to capture the HepG2 cancer cells that pass through the channel. The flushing flow-rate was optimized. By counting the number of cells in the channels using the microscope and evaluating the capture efficiency effectively, in the system, the capture efficiency can reach 64.2%.

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Periodical:

Key Engineering Materials (Volume 483)

Pages:

316-319

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.483.316

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Online since:

June 2011

Authors:

Mei Hang Wei, Yi Xu, Dong Shun Li, Zhen Zhou

Keywords:

Capture Efficiency, C-Met, EDC, HepG2 Cells, Microfluidic Immunoassay Chip

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References

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