Spectroscopic Studies of Adsorbed Myoglobin on Hydroxyapatite Surface

Abstract:

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In this work myoglobin (Mb) adsorption was carried out in a batch system using hydroxyapatite (HA) powder during 24 hours at 37°C. The HA samples were analyzed after protein adsorption by Fourier Transformed Infrared (FTIR) and UV-Vis Spectroscopy in reflectance mode. UV-Vis analyses showed that Soret and Q bands shifted to lower wavelengths when Mb is associated with HA surface. This result suggests that Mb Heme group is sensitive to the protein adsorption onto HA surface. HA disks coated with myoglobin and cultured with human osteoblastic cells during 7 days showed that cell adhesion and proliferation were not inhibited by the protein coating after 7 days in cell culture.

Info:

Periodical:

Key Engineering Materials (Volumes 493-494)

Main Theme:

Edited by:

Eyup Sabri Kayali, Gultekin Goller and Ipek Akin

Pages:

504-507

DOI:

10.4028/www.scientific.net/KEM.493-494.504

Citation:

A. M. Costa et al., "Spectroscopic Studies of Adsorbed Myoglobin on Hydroxyapatite Surface", Key Engineering Materials, Vols. 493-494, pp. 504-507, 2012

Online since:

October 2011

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Price:

$35.00

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