Spectroscopic Studies of Adsorbed Myoglobin on Hydroxyapatite Surface

Andrea Machado Costa; Elena Mavropoulos; Alexandre Mello; Euler A. dos Santos; Renata R. de Oliveira; Marcelo Henrique Prado da Silva; Alexandre Rossi

doi:10.4028/www.scientific.net/KEM.493-494.504

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HomeKey Engineering MaterialsKey Engineering Materials Vols. 493-494Spectroscopic Studies of Adsorbed Myoglobin on...

Spectroscopic Studies of Adsorbed Myoglobin on Hydroxyapatite Surface

Abstract:

In this work myoglobin (Mb) adsorption was carried out in a batch system using hydroxyapatite (HA) powder during 24 hours at 37°C. The HA samples were analyzed after protein adsorption by Fourier Transformed Infrared (FTIR) and UV-Vis Spectroscopy in reflectance mode. UV-Vis analyses showed that Soret and Q bands shifted to lower wavelengths when Mb is associated with HA surface. This result suggests that Mb Heme group is sensitive to the protein adsorption onto HA surface. HA disks coated with myoglobin and cultured with human osteoblastic cells during 7 days showed that cell adhesion and proliferation were not inhibited by the protein coating after 7 days in cell culture.

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Periodical:

Key Engineering Materials (Volumes 493-494)

Pages:

504-507

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.493-494.504

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Online since:

October 2011

Authors:

Andrea Machado Costa, Elena Mavropoulos, Alexandre Mello, Euler A. dos Santos, Renata R. de Oliveira, Marcelo Henrique Prado da Silva, Alexandre Rossi

Keywords:

Hydroxyapatite (HAP), Myoglobin, Spectroscopy

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References

[1] J. L. Brash, T. A. Horbett, Proteins at Interfaces an Overview, ACS Symposium Series, American Chemical Society, Washington, (1995).

Google Scholar

[2] A. Dong, S. J. Prestrelski, S. D. Allison, J. F. Carpenter, Infrared Spectroscopic Studies of Lyophilization- and Temperature-Induced Protein Aggregation, Journal of Pharmaceutical Sciences, 84: 4 (1995) 415- 424.

DOI: 10.1002/jps.2600840407

Google Scholar

[3] K. Kaiden, T. Matsui, S. Tanaka, A Study of the Amide III Band by FT-IR Spectrometry of the Secondary Structure of Albumin, Myoglobin, and "y-Globulin, APPLIED SPECTROSCOPY 41: 2 (1987)180-184.

DOI: 10.1366/000370287774986714

Google Scholar

[4] E.D. Eanes, H.I. Gillessen, A.S. Posner, Nature 23 (1965) 365–367.

Google Scholar

[5] S. Franzen, L. J. Moore, W. H. Woodruff, S. G. Boxer, Stark-Effect Spectroscopy of the Heme Charge-Transfer Bands of Deoxymyoglobin, J. Phys. Chem. B 103 (1999) 3070-3072.

DOI: 10.1021/jp9848288

Google Scholar

[6] B. A. Springer, S. G. Sligar, High-level expression of sperm whale myoglobin in Escherichia coli, Proc. Natl. Acad. Sci. USA 84 (1987) 8961-8965.

DOI: 10.1073/pnas.84.24.8961

Google Scholar

[7] M. Iafisco, B. Palazzo, G. Falini, M. Di Foggia, S. Bonora, S. Nicolis, L. Casella, N. Roveri, Adsorption and Conformational Change of Myoglobin on Biomimetic Hydroxyapatite Nanocrystals Functionalized with Alendronate, Langmuir 24 (2008).

DOI: 10.1021/la703381h

Google Scholar