PVP Addition during Hydroxyapatite Synthesis: Effect on Albumin Adsorption

Abstract:

Hydroxyapatite nanoparticles were synthesized by preparing an aqueous solution comprising 0.5M Ca(OH)₂, 0.3M H₃PO₄ and 1M CH₃CHCO₂HOH (lactic acid) at pH of 3.7. KOH was added to the solution until pH=12 was reached, to precipitate hydroxyapatite. The incubation time ranged from 24 to 88 hours and the precipitates were then filtered on filter paper with the aid of a vacuum pump. The powders were synthesized with and without PVP addition. The powders were then sintered at 1100°C. Sintered and non sintered powders were characterized by scanning electron microscopy with field emission gun (FEG-SEM) and transmission electron microscopy (TEM) in order to assess particles morphology. Structural characterization was performed by X-ray diffractometry with Rietveld refinement. Albumin adsorption experiments were carried out on the powders with the aid of UV-VIS spectrometry. The results showed that the samples produced in the presence of PVP showed smaller particles when compared to non-dispersed samples. Albumin adsorption was more effective on the powders synthesized with dispersant.

• Periodical: Key Engineering Materials (Volumes 493-494)
• Pages: 757-762
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.493-494.757
• Online since: October 2011
• Authors: T.J. Fernandes, E. Mavropoulos, J.B. Campos, L.A. Gobbo, Marcelo Henrique Prado da Silva
• Keywords: Albumin, Hydroxyapatite (HAP), Protein Adsorption, PVP

References

[1] Kong, L. B.; Ma, J.; Boey, F. Nanosized hydroxyapatite powders derived from coprecipitation process. J Materials Science, 37 (2002) 1131-1134.

[2] Borojevic, R. Medicina Regenerativa: terapias celulares, bioengenharia e biomimética. Seminários temáticos para a 3ª Conferência Nacional de C, T&I. Parcerias Estratégicas, n. 20 (2005), 1639-1647.

[3] Sanosh, K. P.; Chu, M-C; Balakrishnan, A; Kim, T. N.; Cho, S-J. Preparation and characterization of nano-hidroxyapatite powder using sol-gel technique. Bull. Mater. Sci., 32, n. 5, (2009) 465–470.

DOI: 10.1007/s12034-009-0069-x

[4] Legeros, R. Z. Calcium Phosphates in Oral Biology and Medicine (Monographs in Oral Science), 15 Basel: Karger, New York, (1991).

[5] Qiu, C. F.; Xiao, X. F.; Liu, R. F.; She, H. D. Biomimetic synthesis of spherical nano-hydroxyapatite with polyvinylpyrrolidone as template. Materials Science and Technology, 24, n. 5 (2008) 612-617.

DOI: 10.1179/174328407x176974

[6] Zhang, Y.; Lu, J. A mild and efficient biomimética synthesis of rodlike hydroxyapatite particles with a high aspect ratio using polyvinylpyrrolidone as capping agent. Crystal growth & Design, 8, n. 7(2008) 2101-2107.

DOI: 10.1021/cg060880e

[7] Mavropoulos, E.; Costa, A. M.; Costa, L. T.; Achete, C. A.; Mello, A.; Granjeiro, J. M.; Rossi, A. M. Adsorption and bioactivity studies of albumin onto hydroxyapatite surface. Colloids and Surfaces B: Biointerfaces, 83 2011 pp.1-9.

DOI: 10.1016/j.colsurfb.2010.10.025