Titanium dioxide is a material widely used in electronics industry and little explored in the biomedical area, which is the objective of this work. Nowadays one can find surgical instruments coated with thin films that have bactericidal properties when they are activated in the presence of ultraviolet light. For crystalline phase control TiO2 was calcinated at 500°C. The crystallite mean size for sample calcinated at 500°C was 27nm. With the results of cytotoxicity it is possible to say that biomedical applications are possible. Electron microscopy images showed nanoparticles obtained by sol-gel process and the compounds were identified by FTIR analysis. Raman spectroscopy confirmed the existence of anatase titania phase and X-ray diffraction showed this material to be composed of a crystalline phase. X-ray fluorescence identified chemical contaminants.