A Hybrid-Structured Microfluidic Chip Developed for ATP Bioluminescence Detection

Abstract:

A hybrid-structured microfluidic chip integrating optical fiber sensing technology and bioluminescence chemical reaction mechanism was developed for rapid and sensitive detection of adenosine 5’-triphosphate (ATP). This microfluidic chip was fabricated with five distinct layers for the successive steps of sample-enzyme reagent mixing, bioluminescence chemical reaction and scatting light collection. The experimental results demonstrated that the provided hybrid-structure improved the sensitivity, response time, reagent consumption and other properties of chip. This improvement enables the microfluidic chip to attain a sensitive ATP detection in the range from 10^-9 to 10^-4 M with excellent linearity (R² = 0.9967), low detection error (CV < 15%), and tiny reagent consumption (< 20 μL). These achievements thus indicated that the chip developed in this study possess the advantages such as high sensitivity, quick-response and small reagent consumption.

• Periodical: Key Engineering Materials (Volumes 531-532)
• Pages: 563-569
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.531-532.563
• Online since: December 2012
• Authors: Wu Ming Zhang, Zhen Yu Li, Tao Chen, Jian Jiang Yao, Yang Lv, Min Li, Guang Li
• Keywords: ATP, Bioluminescence Detection, Biosensor, Fiber Optics, Micro Mixer, Microfluidic Chip

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