Paper Titles
The Precise Measurements of Coercivity Fields of Nd-Fe Ferro-Magnets in Pulsed High Magnetic Fields up to 20T
p.483
Parameters Consideration in Designing a Magnetorheological Damper
p.487
Optimization and Application of NTC Thick Film Segmented Thermistors
p.491
Stress Dependence of Switching Field during the Devitrification of Finemet-Based Magnetic Microwires
p.495
MIP-Based Impedimetric Sensors
p.499
Evaluation of the Polarization State of Integrated Piezoelectric Sensors and Actuators Using the Thermal Wave Method
p.503
Frequency Dependence of Complex Permeability of Polycrystalline Ferrites Based on the Realities of Microstructure
p.507
Full Factorial Design to Study Material Parameters of Magnetorheological Fluid
p.511
Piezoelectric Immunosensors for Toxins' Detection in Foodstuffs
p.515

Piezoelectric Immunosensors for Toxins' Detection in Foodstuffs

Article Preview

Abstract:

The procedures of detecting trace concentrations of okadaic acid and staphylococcal enterotoxin A in foodstuffs by the piezoelectric immunosensors have been developed. The conditions for immobilizing antibodies to toxins on the surface of the sensors gold electrode have been facilitated. The calibration curves for the detection of okadaic acid and staphylococcal enterotoxin A are linear in the range of concentrations 5500 ng·ml-1 and 1-80 ng·ml-1, the limits of detection are 1.4 and 0.4 ng·ml-1 correspondingly. The time of analysis does not exceed 30 min. The sensors were tested in detecting okadaic acid in fish products and in detecting staphylococcal enterotoxin A in chicken meat and milk.

You might also be interested in these eBooks
Materials and Applications for Sensors and Transducers II View Preview

Info:

Periodical:

Key Engineering Materials (Volume 543)

Pages:

515-518

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.543.515

Citation:

Cite this paper

Online since:

March 2013

Authors:

N.A. Karaseva, T.N. Ermolaeva

Keywords:

Bacteriotoxin, Monoclonal Antibodies, Phycotoxin, Piezoelectric Immunosensor

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2013 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

References

[1] Marjorie B. Medina. A biosensor method for detection of staphylococcal enterotoxin A in raw whole egg. Jiornal of rapid methods and automation in microbiology. V. 14 (2006) pp.119-132.

DOI: 10.1111/j.1745-4581.2006.00035.x

Google Scholar

[2] Alice X.J. Tang, Miloslav Pravda, George G. Guilbault at all. Immunosensor for okadaic acid using quartz crystal microbalance. Analytica Chimica Acta. 471 (2002). pp.33-40.

DOI: 10.1016/s0003-2670(02)00922-4

Google Scholar

[3] T. N. Ermolaeva, E. N. Kalmykova. Piezoelectric immunosensors: analytical potentials and outlooks, RUSS CHEM REV, 75 (5) (2006) p.397–409.

DOI: 10.1070/rc2006v075n05abeh001206

Google Scholar

[4] Olimpia Pepe, Giuseppe Blaiotta, Francesca Bucci, Marilena Anastasio, Maria Aponte, and Francesco Villani. Staphylococcus aureus and Staphylococcal Enterotoxin A in Breaded Chicken Products: Detection and Behavior during the Cooking Process. Appl Environ Microbiol. 72(11) (2006).

DOI: 10.1128/aem.00198-06

Google Scholar

[5] Marcaillou-Le Baut C, Amzil Z, Vernoux JP, Pouchus YF, Bohec M, Simon JF. Studies on the detection of okadaic acid in mussels: preliminary comparison of bioassays. Nat Toxins. 2(5) (1994) pp.312-317.

DOI: 10.1002/nt.2620020510

Google Scholar

[6] Ji Hoe Kim, Toshiyuki Suzuki, Ka Jeong Lee, Poong Ho Kim, Takashi Kamiyama, Tae Seek Lee. The first detection of okadaic acid and its derivatives in Korean bivalves by liquid chromatography-mass spectrometry. Fisheries Scienc. V. 74. Issue 2 (2008).

DOI: 10.1111/j.1444-2906.2008.01541.x

Google Scholar

[7] Karaseva N.A., Ermolaeva T.N. A piezoelectric immunosensor for chloramphenicol detection. Talanta. V. 93 (2012) pp.44-48.

DOI: 10.1016/j.talanta.2011.12.047

Google Scholar

[8] Joseph K. H. Ma, H. W. Jun, L. A. Luzzi. Determination of equilibrium constants and binding capacities using a modified scatchard method in drug-protein binding studies. Journal of Pharmaceutical Sciences. V. 62, Issue 12 (1973) p.2038–(2040).

DOI: 10.1002/jps.2600621233

Google Scholar