Microdevice-Based DNA Extraction Method Using Green Reagent

Abstract:

In this paper, a novel microdevice-based solid phase extraction (SPE) method has been developed to extract genomic DNA from real biological samples, such as whole blood, by using a green reagent NaCl as the new binding salt. Guanidine as a bind salt was widely used for extraction DNA with a high extraction efficiency. But it was proved that guanidine was a kind of polymerase chain reaction (PCR) inhibitors, which is also toxic and the waste of guanidine might contaminate environment. NaCl is a natural, nontoxic, cheap, and green reagent, which would be a perfect candidate to replace guanidine. Silicon - polydimethylsiloxane (PDMS) - glass microdevice with a microchannel was designed and fabricated by micro electromechanical system (MEMS) technology. The DNA adsorption on the surface of the microchannel was observed by scan electron microscopy (SEM) technology. Then both NaCl and guanidine were used as the binding salt to extract genomic DNA from real biological sample. Compared with the binding salt of guanidine, the microdevice-based SPE method using NaCl is able to extract enough genomic DNA from rat whole blood in 30 min. About 725.2-1279.3 ng/mL genomic DNA was successfully extracted from whole blood by using NaCl. This highly efficient, effortless, and green method can be widely used as a lab-on-a-chip component for initial biologic sample preparation.

• Periodical: Key Engineering Materials (Volumes 562-565)
• Pages: 1111-1115
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.562-565.1111
• Online since: July 2013
• Authors: Xing Chen, Da Fu Cui, Jian Hai Sun, Lu Lu Zhang, Hui Li
• Keywords: DNA Purification, Micro-Electromechanical System (MEMS), Micro-Fluidics, Solid-Phase Extraction (SPE), Whole Blood

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