Specific Detection of Staphylococcus Enterotoxin B in Milk Using Piezoresistive Cantilever Aptasensor

Abstract:

In this paper, an aptasensor with high sensitivity and rapid response was developed for the detection of staphylococcus enterotoxin B (SEB) by using thiol-modified piezoresistive cantilever. Thiol-modified aptamers, acting as the functionalized sensing elements, were immobilized on the nanogold-coated surface of the sensing cantilever as agents for detecting SEB. By using the functionalized aptasensors, different concentrations of SEB were detected with a wide detection range of 6-100 ng/mL and a quick response in milk. The experimental results indicated that the cantilever-based aptasensors had sufficient sensitivity for the detection of SEB in real food commodities and might provide an economical platform for on-site detections of different toxicants with the advantages of portability, high sensitivity, and rapid response.

• Periodical: Key Engineering Materials (Volumes 645-646)
• Pages: 1298-1302
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.645-646.1298
• Online since: May 2015
• Authors: Rui Zhao, Yong Zheng Wen, Wei Ma, Jian Cheng Yang, Xiao Mei Yu*
• Keywords: Aptamers, Aptasensor, Piezoresistive Cantilevers, Staphylococcus Enterotoxin B (SEB)

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