Separation of Non-Natural Carboxyl Amino Acid Enantiomers by Capillary Electrophoresis with CM-β-Cyclodextrins as Chiral Selective Reagent

Abstract:

A method was built to separate three kinds of enantiomers of non-natural carboxyl amino acid with CE while CM-β-CD acted as chiral selective reagent. Several different β-CD derivatives were used as the chiral separation agent, and it was proved that CM-β-CD had the advantage of chiral selectivity for carboxyl amino acids. Three kinds of enantiomers of carboxyl amino acids were separated by CE in a 50 μm i.d.×60 cm (effective length 45 cm) fused-silica capillary at 20 kV voltages and 10 mM NaH₂PO₄ solution served as running buffer solution with 10 mM CM-β-CD serving as selective reagent. The optimum detection wavelength was 214nm.

• Periodical: Key Engineering Materials (Volume 723)
• Pages: 540-544
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.723.540
• Online since: December 2016
• Authors: Bao Hui Li*, Xiao Yan Li, Xiao Dan Yang
• Keywords: Capillary Electrophoresis, Carboxymethyl-β-Cyclodextrin, Non-Natural Amido Amino Acid

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