Isolation, Identification, and Characterization of Lambda-Cyhalothrin Pesticide Degrading Bacterium ZC-5

Abstract:

A highly efficient lambda-cyhalothrin-degrading bacterium, designated as strain ZC-5, was isolated from the activated sludge of a sewage aeration tank in a pesticide factory by enrichment acclimation and the streak plate method. Strain ZC-5 can grow on minimal medium with lambda-cyhalothrin as the sole source of carbon and nitrogen. After cultivation for 6 h to 24 h, the biomass of the bacterial strain significantly increased at the logarithmic phase. By contrast, the concentration of lambda-cyhalothrin rapidly decreased. The residual lambda-cyhalothrin presented a concentration of 250 mg/L and a degradation rate of 50%. Gas chromatography revealed that this strain can degrade 87.1% lambda-cyhalothrin (500 mg/L) in the culture within 2 days. Morphological analysis showed the Gram-negative strain as short rods. Physiological and biochemical characterizations, as well as phylogenetic analysis of the 16S rDNA sequence identified the bacterium to be an Achromobacter xylosoxidans strain. Results showed that this strain can provide a novel strategy to biodegrade the pesticide lambda-cyhalothrin.

• Periodical: Key Engineering Materials (Volume 723)
• Pages: 628-632
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.723.628
• Online since: December 2016
• Authors: Rong Hu Zhang, Zhen Hua Zhou, Jian Cheng Feng*
• Keywords: Achromobacter xylosoxidans, Degrading Bacterium, Identification, Isolation, Lambda-Cyhalothrin, Organic Materials

References

[1] Y. Shukla, A. Yadav, A. Arora, Carcinogenic and cocarcinogenic potential of cypermethrin on mouse skin, Cancer. Lett. 182 (2002) 33-41.

DOI: 10.1016/s0304-3835(02)00077-0

[2] P. N. Saxena, L. K. Chauhan, S. K. Gupta, Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: spectroscopic basis of chromosome damage, Toxicol. 216 (2005) 244-252.

DOI: 10.1016/j.tox.2005.08.008

[3] P. Pandey, G. Pant, G. Sibi, Isolation and characterization of bifenthrin catabolizing bacterial strain Bacillus cibi from soil for pyrethroids biodegradation, Online J. Biol. Sci. 14 (2014) 188.

DOI: 10.3844/ojbsci.2014.188.195

[4] S. Chen, Y. Deng, C. Chang, J. Lee, Y. Cheng, Z. Cui, J. Zhou, F. He, M. Hu, L. H. Zhang, Pathway and kinetics of cyhalothrin biodegradation by Bacillus thuringiensis strain ZS-19, Sci. Rep. 5 (2015) 8784.

DOI: 10.1038/srep08784

[5] M. R. McCoy, Z. Yang, X. Fu, K. C. Ahn, S. J. Gee, D. C. Bom, Monitoring of total type II pyrethroid pesticides in citrus oils and water by converting to a common product 3-phenoxybenzoic acid , J. Agric. Food Chem. 60 (2012) 5065.

DOI: 10.1021/jf2051653

[6] H. Harms, D. Schlosser, L. Y. Wick, Untapped potential: exploiting fungi in bioremediation of hazardous chemicals, Nat. Rev. Microbiol. 9 (2011) 177-192.

DOI: 10.1038/nrmicro2519

[7] J. C. Feng, Selection of a mutant strain Streptococcus equi SH-109 and optimization of culture conditions for the production of high molecular weight hyaluronic acid, Adv. Mater. Res. 915-916 (2014) 909-912.

DOI: 10.4028/www.scientific.net/amr.915-916.909

[8] G. P. Hu, F. Q. Song, B. Liu, Y. Zhao, M. S. You, Isolation, identification and degradation characteristics of Cyfluthrin-degrading strain FLQ-5, Chin. J. Appl. Environ. Biol. 19 (2013): 851-856.

DOI: 10.3724/sp.j.1145.2013.00851

[9] S. H. Chen, L. Yang, M. Y. Hu, J. J. Liu, Biodegradation of fenvalerate and 3-phenoxybenzoic acid by a novel Stenotrophomonas sp. strain ZS-S-01 and its use in bioremediation of contaminated soils, Appl. Microbiol. Biotechnol. 90 (2011) 755-767.

DOI: 10.1007/s00253-010-3035-z

[10] W. Q. Liang, Z. Y. Wang, H. Li, P. C. Wu, M. Hu, N. Luo, L. X. Cao, Y.H. Liu, Purification and characterization of a novel pyrethroid hydrolase from Aspergillus niger ZD11, J. Agric. Food Chem. 53 (2005) 7415-7420.

DOI: 10.1021/jf051460k