Direct Methanolysis of Oleaginous Yeast Biomass (Pseudozyma parantarctica) to Microbial Biodiesel

Atsdawut Areesirisuk; Chiu Hsia Chiu; Tsair Bor Yen; Jia Hsin Guo

doi:10.4028/www.scientific.net/KEM.753.259

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HomeKey Engineering MaterialsKey Engineering Materials Vol. 753Direct Methanolysis of Oleaginous Yeast Biomass...

Direct Methanolysis of Oleaginous Yeast Biomass (Pseudozyma parantarctica) to Microbial Biodiesel

Abstract:

In this study, intracellular lipids of a novel oleaginous biomass of P. parantarctica were converted to biodiesel directly using simple acid catalyst methanolysis. The optimum condition of this method was investigated. Under optimum conditions (0.1 M H₂SO₄, 10 h reaction time, 65°C reaction temperature, and 1:20 (w/v) biomass-to-methanol ratio), the yield of crude biodiesel was 93.18 ± 2.09% based on total cellular lipids. The composition of crude biodiesel was C16:C18 fatty acid methyl esters (FAMEs) for 91.91%. Especially, the C18:1 methyl ester was the main FAME (47.10%). In addition, the result showed that this technique could produce the microbial biodiesel from biomass containing high free fatty acids (FFAs) without soap formation. The predicted cetane number and kinematic viscosity of biodiesel were characterized according to ASTM D6751 and EN 14214 standards. Our results indicated that this process produces a good quality biodiesel. Moreover, it can decrease the manufacturing costs of microbial biodiesel production from oleaginous yeast biomass without cell disruption and lipid extraction.

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Key Engineering Materials (Volume 753)

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259-263

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https://doi.org/10.4028/www.scientific.net/KEM.753.259

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August 2017

Authors:

Atsdawut Areesirisuk*, Chiu Hsia Chiu, Tsair Bor Yen, Jia Hsin Guo

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Direct Methanolysis, Microbial Biodiesel, Oleaginous Yeast, Pseudozyma parantarctica

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