Comparison of Supermacroporous Polyester Matrices Fabricated by Thermally Induced Phase Separation and 3D Printing Techniques

Abstract:

Supermacroporous three-dimensional matrices based on poly-D,L-lactide or polycaprolactone were fabricated by thermally induced phase separation method and 3D printing technique. The morphology and mechanical properties of the resulting matrices were studied with the use of optical and scanning electron microscopy and the uniaxial compression test, respectively. All matrices were characterized with supermacroporous structure suitable for cell penetration. A significant increase in Young's modulus and tensile strength was established for both polymer matrices prepared by 3D printing technique.

• Periodical: Key Engineering Materials (Volume 822)
• Pages: 277-283
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.822.277
• Online since: September 2019
• Authors: Mariia Stepanova, Aleksei Eremin, Ilia Averianov, Iosif Gofman, Antonina Lavrentieva, Viktor Korzhikov-Vlakh, Evgenia Korzhikova-Vlakh*
• Keywords: 3D Printing, Mechanical Properties, Polycaprolactone, Poly-D,L-Lactide, Polyesters, Scaffolds, Supermacroporous Polymeric Matrix, Thermally Induced Phase Separation, Uniaxial Compression

References

[1] P.V. Popryadukhin, G.Y. Yukina, E.M. Dobrovolskaya, I.P. Ivankova, V.E. Yudin, Bioresorption of Porous 3D Matrices Based on Collagen in Liver and Muscular Tissue, Cell Tissue Biol. 12 (2018) 247–255.

DOI: 10.1134/s1990519x18030094

[2] J.A. Stella, A. D'Amore, W.R. Wagner, M.S. Sacks, On the biomechanical function of scaffolds for engineering loadbearing soft tissues, Acta Biomater . 6 (2010) 2365–2381.

DOI: 10.1016/j.actbio.2010.01.001

[3] N.M. Yudintceva, Y.A. Nashchekina, M.I. Blinova, M.A. Shevtsov, N.V. Orlova, A.N. Muraviov, T.I. Vinogradova, M.G. Sheykhov, E.Y. Shapkova, D.V. Emeljannikov, I.A. Yablonskii, P.K. Samusenko, A.L. Mikhrina, A.V. Pakhomov, Experimental bladder regeneration using a poly-L-lactide/silk fibroin scaffold seeded with nanoparticle-labeled allogenic bone marrow stromal cells, Int. J. Nanomedicine. 11 (2016) 4521–4533.

DOI: 10.2147/ijn.s111656

[4] X. Zhang, X. Li, H. Fan, X. Liu, Preparation and Characterization of Porous β-TCP/PLLA Composites with High β-TCP Content, Key Eng. Mater. 330–332 (2007) 491–494.

DOI: 10.4028/www.scientific.net/kem.330-332.491

[5] G.I. Popov, A.E. Kryukov, P.V. Popryadukhin, Y.A. Naschekina, E.M. Ivankova, V.N. Vavilov, N.V. Smirnova, Optimal Methods of Cell Seeding and Cultivation on a Poly(L-lactide) Biodegradable Scaffold, Cell Tissue Biol. 12 (2018) 359–366.

DOI: 10.1134/s1990519x1805005x

[6] K.V. Malafeev, O.A. Moskalyuk, V.E. Yudin, V.Y. Elokhovskii, E.N. Popova, L.S. Litvinova, D.N. Suslov, E.M. Ivan'kova, Synthesis and properties of fibers based on polylactide stereocomplexes, Rus. J. Appl. Chem. 90 (2017) 1021–1029.

DOI: 10.1134/s1070427217070011

[7] V.V. Matrenichev, P.V. Popryadukhin, A.E. Kryukov, N.V. Smirnova, E.M. Ivan'kova, I.P. Dobrovol'skaya, V.E. Yudin, Properties of Film Materials Based on Composite Nanofibers from Aliphatic Copolyamide and Carbon Nanotubes for Tissue Engineering, Polym. Sci. Ser. A. 60 (2018) 215–221.

DOI: 10.1134/s0965545x18020104

[8] G. Chen, T. Ushida, T. Tateish, Scaffold design for tissue engineering., Macromol Biosci. 2 (2002) 67–77.

[9] Y.A. Nashchekina, D.M. Darvish, M.I. Blinova, K.E. Kurdyukova, I.M. Zorin, A.Y. Bilibin, E.S. Tsobkallo, Synthesis of D,L-lactide–ε-caprolactone copolymers and preparation of films based on them, Rus. J. Appl. Chem. 91 (2018) 113–120.

DOI: 10.1134/s1070427218010184

[10] B.D. Ulery, L.S. Nair, C.T. Laurencin, Biomedical Applications of Biodegradable Polymers, J Polym Sci B Polym Phys. 49 (2011) 832–864.

DOI: 10.1002/polb.22259

[11] Y. Wang, Q. Wen, H.S. Choi, FDA's Regulatory Science Program for Generic PLA/ PLGA-Based Drug Products, Am. Pharm. Rev. (2016) ID 188841.

[12] K.J. Cho, D.K. Song, S.H. Oh, Y.J. Koh, S.H. Lee, M.C. Lee, J.H. Lee, Fabrication and Characterization of Hydrophilized Polydioxanone Scaffolds for Tissue Engineering Applications, Key Eng. Mater. 342–343 (2007) 289–292.

DOI: 10.4028/www.scientific.net/kem.342-343.289

[13] B. Azimi, P. Nourpanah, M. Rabiee, S. Arbab, Poly (ε-caprolactone) Fiber: An Overview, J. Eng. Fiber. Fabr. 9 (2014) 74–90.

DOI: 10.1177/155892501400900309

[14] F. Shady, Physical and Mechanical Properties of PLA, and Their Functions in Widespread Applications — A Comprehensive Review, Adv. Drug Deliv. Rev. 107 (2016) 367–392.

[15] C. Lao, C. Chen, J. Chen, S. Chiang, Y. Lin, K. Chang, Fabrication of porous biodegradable polymer scaffolds using a solvent merging/particulate leaching method, J. Biomed. Mater. Res. 59 (2002) 676–681.

DOI: 10.1002/jbm.10030

[16] N.V. Smirnova, N.O. Petrova, I.O. Lebedeva, P.V. Popryadukhin, V.E. Yudin, Tissue reconstruction of skin failures and soft-tissue injuries using regenerative medicine methods, St. Petersbg. Polytech. Univ. J. Phys. Math. 2 (2016) 322–328.

DOI: 10.1016/j.spjpm.2016.11.008

[17] P.V. Popryadukhin, G.I. Popov, G.Y. Yukina, I.P. Dobrovolskaya, E.M. Ivan'kova, V.N. Vavilov, V.E. Yudin, Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers, Int. J. Biomater. (2017) 1–10.

DOI: 10.1155/2017/9034186

[18] G.I. Popov, A.E. Kryukov, P. V. Popryadukhin, Y.A. Naschekina, E.M. Ivankova, V.N. Vavilov, V.E. Yudin, N. V. Smirnova, Determining optimal methods of cell seeding and cultivation on L-polylactide biodegradable scaffold, Cell Tissue Biol. 60 (2018) 279–286.

DOI: 10.1134/s1990519x1805005x

[19] E. Nejati, H. Mirzadeh, M. Zandi, Synthesis and characterization of nanohydroxyapatite rods/poly(L-lactide acid) composite scaffolds for bone tissue engineering, Compos. A. 39 (2008) 1589–1596.

DOI: 10.1016/j.compositesa.2008.05.018

[20] S. Singare, S.Y. Zhong, Z.Z. Sun, A Method to Fabricate Liver Tissue Engineering Scaffold, J. Biomimetics, Biomater. Tissue Eng. 11 (2011) 73–80.

DOI: 10.4028/www.scientific.net/jbbte.11.73

[21] T.D. Roy, J.L. Simon, J.L. Ricci, E.D. Rekow, V.P. Thompson, J.R. Parsosns, Engineered cellular response to scaffold architecture in a rabbit trephine defect, J. Biomed. Mater. Res. 66A (2003) 283–291.

DOI: 10.1002/jbm.a.10569

[22] D. Adel-Khattab, F. Giacomini, B. Peleska, R. Gildenhaar, G. Berger, C. Gomes, U. Linow, M. Hardt, J. Günster, A. Houshmand, M. Stiller, K.A. Ghaffar, A. Gamal, M. El-Mofty, C. Knabe, Development of a Synthetic Tissue Engineered 3D Printed Calciumalkaliphosphate-Based Bone Graft with Homogenously Distributed Osteoblasts and Mineralizing Bone Matrix In Vitro, Key Eng. Mater. 720 (2017) 82–89.

DOI: 10.4028/www.scientific.net/kem.720.82

[23] N.K. Bawolin, M.G. Li, X.B. Chen, W.J. Zhang, Modeling material-degradation-induced elastic property of tissue engineering scaffolds, J Biomech Eng. 132 (2010) 111001-111007.

DOI: 10.1115/1.4002551

[24] I. V. Averianov, V.A. Korzhikov-Vlakh, Y.E. Moskalenko, V.E. Smirnova, T.B. Tennikova, One-pot synthesis of poly(lactic acid) with terminal methacrylate groups for the adjustment of mechanical properties of biomaterials, Mendeleev Commun. 27 (2017) 574–576.

DOI: 10.1016/j.mencom.2017.11.012