Preparation and Evaluation of 6-Maleimidohexanoic Acid Grafted Chitosan Nanoparticles as a Novel Carrier for Intranasal Protein Delivery

Abstract:

In this study, 6-maleimidohexanoic acid grafted chitosan nanoparticles (MHA-CS NPs) were prepared and evaluated the efficiency of intranasal protein delivery as compared with well-known chitosan nanoparticles (CS NPs). Fluorescein isothiocyanate labelled with bovine serum albumin (FITC-BSA) was used as a model protein. The results indicated that both CS NPs and MHA-CS NPs were positively charged NPs before and after protein loading. The condition for optimal protein loading was 1:6 mass ratio of protein/NPs at 1 h incubation period. The optimal formulations of CS NPs and MHA-CS NPs were evaluated on porcine mucosa as ex vivo. The mucoadhesive and permeation properties of FITC-BSA loaded MHA-CS NPs showed a greater than FITC-BSA loaded CS NPs and FITC-BSA solution, respectively. These ex vivo studies present the potential of MHA-CS NPs as a novel carrier for intranasal protein delivery that will be a candidate for in vivo study.

• Periodical: Key Engineering Materials (Volume 859)
• Pages: 214-219
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.859.214
• Online since: August 2020
• Authors: Ekachai Dumkliang, Tanasait Ngawhirunpat, Theerasak Rojanarata, Prasopchai Patrojanasophon, Boonnada Pamornpathomkul, Praneet Opanasopit*
• Keywords: Chitosan, Intranasal Delivery, Nanoparticles, Protein

References

[1] A.C. Anselmo, Y. Gokarn, S. Mitragotri, Non-invasive delivery strategies for biologics, Nat Rev Drug Discov. 18 (2019) 19-40.

DOI: 10.1038/nrd.2018.183

[2] M.A. Mohammed, J.T.M. Syeda, K.M. Wasan, E.K. Wasan, An overview of chitosan nanoparticles and its application in non-parenteral drug delivery, Pharmaceutics. 9 (2017) e53.

DOI: 10.3390/pharmaceutics9040053

[3] N. Sahatsapan, T. Rojanarata, T.Ngawhirunpat, P. Opanasopit, P. Tonglairoum, 6-Maleimidohexanoic acid -grafted chitosan: A new generation mucoadhesive polymer, Carbohydr Polym. 202 (2018) 258-264.

DOI: 10.1016/j.carbpol.2018.08.119

[4] D. Bento, S. Jesus, F. Lebre, T. Goncalves, O. Borges, Chitosan plus compound 48/80: formulation and preliminary evaluation as a Hepatitis B vaccine adjuvant, Pharmaceutics. 11 (2019) e72.

DOI: 10.3390/pharmaceutics11020072

[5] D.B. Kaldybekov, P. Tonglairoum, P. Opanasopit. V.V. Khutoryanskiy, Mucoadhesive maleimide-functionalised liposomes for drug delivery to urinary bladder, Eur J Pharm Sci. 111 (2018) 83-90.

DOI: 10.1016/j.ejps.2017.09.039

[6] P. Mura, N. Mennini, C. Nativi, B. Richichi, In situ mucoadhesive-thermosensitive liposomal gel as a novel vehicle for nasal extended delivery of opiorphin, Eur J Pharm Biopharm. 122 (2018) 54-61.

DOI: 10.1016/j.ejpb.2017.10.008

[7] A. Ali, M. Wahlgren, B. Rembratt-Svensson, A. Daftani, P. Falkman, P. Wollmer, et al, Dehydration affects drug transport over nasal mucosa, Drug Deliv. 26 (2019) 831-840.

DOI: 10.1080/10717544.2019.1650848