Electrospinning of Eudragit RS100 for Nerve Tissue Engineering Scaffold

Abstract:

Electrospinning technique is widely investigated in medical applications such as tissue engineering scaffolds, wound dressing and drug delivery. In this study, the aligned nanofiber scaffold of Eudragit RS100 was successfully fabricated via electrospinning technique for nerve tissue engineering scaffold. The diameter distribution and degree of alignment of Eudragit RS100 nanofiber scaffold were observed by scanning electron microspore (SEM). The chemical and crystalline structure of Eudragit RS100 nanofiber scaffold were analyzed using Fourier transform infrared spectroscopy (FTIR) and Powder X-ray diffactometer (PXRD). Cell culture studies using rat Schwann cells were determined to evaluate cell proliferation cell alignment and morphology. The results implied that the diameter of fiber was in the nanometer region. The Eudragit RS100 nanofiber scaffold were in an amorphous form and its chemical structure was not destructive after the electrospinning process. The Eudragit RS100 nanofiber scaffold showed biocompatibility with rat Schwann cells and growing parallel to the aligned fibers. In conclusion, the Eudragit RS100 nanofiber scaffold may have the ability to apply to nerve tissue engineering scaffold.

• Periodical: Key Engineering Materials (Volume 859)
• Pages: 220-225
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.859.220
• Online since: August 2020
• Authors: Natthan Charernsriwilaiwat*, Thapakorn Chareonying, Praneet Opanasopit
• Keywords: Electrospinning, Eudragit RS100, Nerve Tissue Engineering, Scaffold

References

[1] Kijeńska, E, M. P. Prabhakaran, W. Swieszkowski, K. J. Kurzydlowski, S. Ramakrishna, Interaction of Schwann cells with laminin encapsulated PLCL core–shell nanofibers for nerve tissue engineering, Eur. Polym. J. 50 (2014) 30-38.

DOI: 10.1016/j.eurpolymj.2013.10.021

[2] X. Gu, F. Ding, D. F. Williams, Neural tissue engineering options for peripheral nerve regeneration, Biomaterials. 35 (2014) 6143-6156.

DOI: 10.1016/j.biomaterials.2014.04.064

[3] E. Sachlos, J. T. Czernuszka, Making tissue engineering scaffolds work. Review on the application of solid freeform fabrication technology to the production of tissue engineering scaffolds, Eur. Cell. Mater. (2003) 29-40.

DOI: 10.22203/ecm.v005a03

[4] A. Cooper, N. Bhattarai, M. Zhang, Fabrication and cellular compatibility of aligned chitosan–PCL fibers for nerve tissue regeneration, Carbohydr. Polym. 85 (2011) 149-156.

DOI: 10.1016/j.carbpol.2011.02.008

[5] S. Pandav, J. Naik, Preparation and In Vitro Evaluation of ethylcellulose and polymethacrylate resins loaded microparticles containing hydrophilic drug, J. Pharm. (Cairo). 2014 (2014) 1-5.

DOI: 10.1155/2014/904036

[6] S. Payab, N. Jafari-Aghdam, M. Barzegar-Jalali, G. Mohammadi, F. Lotfipour, T. Gholikhani, K. Adibkia, Preparation and physicochemical characterization of the azithromycin-Eudragit RS100 nanobeads and nanofibers using electrospinning method, J. Drug. Deliv. Sci. Technol. 24 (2014) 585-590.

DOI: 10.1016/s1773-2247(14)50123-2

[7] N. Charernsriwilaiwat, T. Rojanarata, T. Ngawhirunpat, P. Opanasopit, Aligned electrospun polyvinyl pyrrolidone/poly e-caprolactone nanofiber mats of tissue engineering, Int. J. Nanosci. 15 (2016) 1-7.

DOI: 10.1142/s0219581x16500058