Preparation and Property of Antibacterial Nucleus with the Combination of Nano-Silver and Antibiotics

Abstract:

To improve the yield and quality of pearls in freshwater pearl culture and the survival rates after nucleus implanting surgery, pearl farmers used artificial pearl nuclear transplantation techniques to raise pearls. To address the common problem of Staphylococcus aureus and Escherichia coli infection in oyster farming, a new prophylactic method by using compound antibiotics to prepare the medicine coated pearl nucleus was put forward based on existing research results of the nanosilver antibacterial nucleus. Single-factor experiment, multi-factor experiment, orthogonal experiment, SPSS analysis of variance was used to optimize the antibacterial formulation on the assumption that the contaminated probability of these two pathogenic bacteria was the same. The result showed that the optimal ratio of compound antibiotics was 0.0075g/ml of the flavomycin solution and 0.01g/mL of the terramycin solution; the inhibition zones diameter of both pathogenic bacteria was more than 2.6cm in vitro, which was higher than the nanosilver antibacterial nucleus of 0.9cm in vitro. Indicating that the addition of this compound antibiotic formula for the nanosilver antibacterial nucleus could reduce the usage of antibiotics under the premise of maintaining antibacterial effectiveness, and could preferably inhibit the pathogenic microorganisms in the postoperative infection period. This also indicates that compound antibiotics coated antibacterial nanosilver nucleus would be applied more widely.

• Periodical: Key Engineering Materials (Volume 905)
• Pages: 282-287
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.905.282
• Online since: January 2022
• Authors: Jie Sun, Le Xin Zhou, Xiao Hong Li, Ming Xia*
• Keywords: Antibacterial, Orthogonal Experiment, Pearl Culture, Pearl Nucleus

References

[1] Liang FL, Xie SH and Lin WC 2015 Transactions of Oceanology and Limnology 115-120.

[2] Liang FL, Wang QG and Deng YW 2015 J Zhanjiang Ocean Univ 35(4) 51-55.

[3] Xie SH, Deng YW and Fu S 2013 Zhanjiang Ocean Univ 33(6) 37-41.

[4] Xie SH, Liang FL and Fu S 2013 Chinese Agricultural Science Bulletin 29(2) 31-35.

[5] Wang QG, Xie SH and Deng YW 2018 Journal of Aquaculture 39(4) 50-53.

[6] Li AH 2001 Microbiology China 28(1) 58-63.

[7] Chen HH, Tu CL and Tang Y 2017 Journal of Fisheries of China 41(11) 1766-78.

[8] Xu XJ, Li XH and Ma YF 2019 Journal of Fisheries of China 43(5) 1338-45.

[9] Wang SQ, Wu QC and Li ZQ A breeding method of colored negative ion pearls[P] China CN104855315A 2015-8-26.

[10] Xu P, Ai KT and Zhou JW 2016 Journal of Yangtze University (Natural Science Edition) 13(09) 26-28+37.