Phytochemical Characterization of Ochna integerrima roots and their In Vitro Anti-Tyrosinase Property

Abstract:

This research aimed to evaluate the effects of ethyl acetate (EtOAc), dichloromethane (DCM), and butanol (n-BuOH) extracts from the roots of Ochna integerrima Merr on their in vitro anti-tyrosinase enzyme activity. The phytochemical composition of these extracts was analyzed using GC-MS/MS. The EtOAc extract was particularly rich in phenols (34.38%), followed by fatty acids and derivatives (18.09%), flavonoids and chalcones (16.94%), and steroids (14.20%). Six secondary metabolites were also isolated from the active fraction (EtOAc extract) using column chromatography. Their structures were elucidated through spectroscopic techniques, primarily 1D and 2D NMR, and cross-referenced with existing literature. The findings revealed that the EtOAc extract exhibited the most significant inhibitory activity against the enzyme tyrosinase, with an IC₅₀ value of 0.106 mg/mL. While the DCM and n-BuOH extracts showed inhibitory effects at 0.1 mg/mL concentrations, with inhibition rates of 6.34% and 26.14%, respectively. These results suggest that the root extracts of O. integerrima have potential anti-tyrosinase properties that could be beneficial in the cosmetics industry. Further investigation is required to identify and isolate additional bioactive compounds of interest.

• Periodical: Key Engineering Materials (Volume 999)
• Pages: 3-10
• DOI: https://doi.org/10.4028/p-hF5BGA
• Online since: December 2024
• Authors: Sutarin Preepram, Anupong Joompang, Supachai Jadsadajerm, Santi Phosri, Awat Wisetsai*
• Keywords: Enzyme Inhibition, GC-MS, Ochna integerrima, Ochnaceae, Tyrosinase

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