Spectrophotometric Determination of Sulphamethoxazole Drug by New Pyrazoline Derived from 2,4-Dinitro Phenyl Hydrazine

Abstract:

Simple, sensitive and accurate spectrophotometric methods have been developed for the determination of Sulphamethoxazole (SMZ) drug . This method based on the reaction of Sulphamethoxazole (SMZ) with new organic reagent (BYN) it was prepare by reaction between ethyl acetoacetate with 2,4-Dinitrophenylhydrazine. The azo dye product shows absorption maximum at 435 nm. The linearity ranges of Sulphamethoxazole are (2-14 μg.mL^-1) with molar absorptivity (14412.77 L.mol^-1.cm^-1), Sandell's sensitivity index (0.0175 μg.cm-²) , detection limit and Quantification limit ( 0.057, 0.175 μg / ml) respectively. The results showed there are no interferences of excipients on the determination of the drug. The proposed method has been successfully applied for the determination of sulfanilamide in pure and pharmaceutical formulations.

• Periodical: Materials Science Forum (Volume 1002)
• Pages: 350-359
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.1002.350
• Online since: July 2020
• Authors: Asmaa Ahmed Mohammed Al Rashidy*, Khalid Abdlaaziz Al Badrany, Gassim Mohammed Al Garagoly
• Keywords: Azo Dye, Pyrazoline, Spectrophotometric, Sulphamethoxazole

References

[1] R. Yin Guo, W. Wang, H. Du, J. Zhou, Selective degradation of sulfonamide antibiotics by peroxymonosulfate ….mechanisms, J. Ch. E. 334 (2018 ) 2539-2546.

DOI: 10.1016/j.cej.2017.11.174

[2] H.Khalaf, A. Al-Haidari, S. Dikran & A.Mohammed, Spectrophotometric Determination of Sulfamethoxazole….Condensation Reaction Method . J. U.B. 25(2) (2017) 524-51. ‏‏[3] H. Hasan, Studying of the physical and chemical characteristics …injections". J. K.Ph.Sci. (12) (2017)49-57.

[4] T.Mahmoud, S. Dikran, A. Mohammed, Spectrophotometric Determination of Sulfamethoxazole ….Sodium Nitroprusside, Ibn AL-Haitham J.P.A. Sci. 30(3) (2017) 102-113.

DOI: 10.30526/30.3.1606

[5] S. Darweesh, I. Al-Haidari, A. Mohammed & S. Dikran , Spectrophotometric Determination of SMZ … Condensation Reaction". Ibn AL-Haitham J.P.A. Sci . 29(2) (2017) 185-199.

[6] O. Adegoke, C. Babalola, O.Kotila, Simultaneous spectrophotometric determination of …. with chloranilic acid". A.J. Ch .10 (2017) S3848-S3860. ‏‏[7] Th. AL-ghabsha, N.theiaa', S.AL-enizzi, Spectrophotometric determination of Sulphamethoxazole .. Reaction". J. Edu. Sci.26.4(2013)56-67.

DOI: 10.1016/j.arabjc.2014.05.022

[8] H.Khalaf , New Spectrophotometric Analytical … Study" Ph.D. Thesis in Chemistry, College of Education for Pure Science Ibn AI- Haitham University of Baghdad (2014).

[9] J. Medinaa, Simultaneous Determination of Trimethoprim and ….Studies" I.J. Ph. and Ph.Sci.5(2013)505- 510.

[10] M. Wang, H. Chang, Analysis of Sulfonamides by …. Liquid‐Phase Microextraction, J.Ch Ch.So .60(12) (2013)1479-1483.

[11] W. Pietroń, W. Cybulski, Determination of five sulfonamides in … ultraviolet detection". B.V.I. 57(4) (2013) 545-552.

[12] T. Catelani, I.Tóth, J. Lima, A simple and rapid screening method for … capillary cell , Talanta, 121(2014), 281-287.

DOI: 10.1016/j.talanta.2013.12.034

[13] R.Injac, J .Kac ,K.Rajic and B.Strukelj , Optimal Condition for extraction and simultaneous determination of Sulfamethoxazol ….Capillary Chromatography , J. F. D. Analysis, 16(1) (2008)18-25.

DOI: 10.38212/2224-6614.2381

[14] D.Agbaba ,A.Radovic ,S.Vladimirov and D.Zivanov-Stakic ,Simultaneous TLC .. Acid in pharmaceuticals , J. Chr.Sci. , 34(199) 460-465.

DOI: 10.1093/chromsci/34.10.460

[15] R.Injac, N.Kocevar and B.Strukelj , Optimized Method for determination of Amoxicillin, Ampicillin, Sulfamethoxazole ….. Liquid Chromatography, Croat. Chem. Acta 82 (3) (2009) 685–694.

[16] H. Givianrad & M. Mohagheghian , Net Analyte Signal Standard Additions Method for Simultaneous Determination of Sulfamethoxazole …Fluids , J. of Ch. 9(2) (2012) 680-692.

DOI: 10.1155/2012/274396

[17] F.Shamsa and L.Amani , Determination of Sulfamethoxazole …Spectrophotometry , Ira. J. Ph.R. ,5(1) (2006) 31-36.

[18] O.Abdelhafez , N. Abdel-Latif , F.Badria , DNA, Antiviral Activities …Benzofuran Derivatives , Ar. Ph.R. , 34(10) (2011)1623-1632.

DOI: 10.1007/s12272-011-1006-2

[19] N.Abdel-Latif, Synthesis and Antidepressant Activity of Some New Coumarin Derivatives , Sci. Ph., 74(2005) 173-216.

DOI: 10.3797/scipharm.aut-05-15

[20] G. AL – Garagoly, A. AL – Rashidy and Kh. AL – Badrany, Spectrophotometric Determination of Sulfanilamide … from 2,4-Dinitro phenyl Hydrazine DJPS 15(4) (October 2019) ,15-3.

DOI: 10.4028/www.scientific.net/msf.1002.350

[21] S. Mohammed, & H.Zamel, ,Spectrophotometric Assay of Sulphamethoxazole in Pure and Pharmaceutical Dosage Forms by Diazotization and Coupling Reaction . RJS 26(1E) (2017) 111-121. ‏‏ ‏‏‏.

DOI: 10.33899/rjs.2017.139119

[22] H. Khalaf, A.Al-Haidari, S. Dikran, Spectrophotometric Determination of Sulfamethoxazole Following Simple Diazotization and Coupling with Diphenylamine, Ibn AL-Haitham J.P.A. Sci, 27(3) (2014)365-380.