Numerical Study of Single Molecular Charge Sensing by FET-Integrated Nanopore Biosensor

Abstract:

This report studies the charge-based sensing modality of FET-embedded nanopore biosensors through FEM simulation. PNP equation is solved to analyze the mirror charge introduced by charged biomolecule while threading through the nanopore-FET sensor. Negative and positive charged molecules are analyzed respectively. Obvious local potential change induced by the presenting of charged molecules nearby is observed. In addition, the transport-induced descreening effect is observed under intensive bias, which might explain the capability of charge sensing even under high concentrations such as 1 M for FET-nanopore biosensors.

• Periodical: Materials Science Forum (Volume 1058)
• Pages: 99-104
• DOI: https://doi.org/10.4028/p-8kmke2
• Online since: April 2022
• Authors: Xin Zhu*, Xiao Jie Li, Yang Liu, Xi Shan Guo, Yin Fei Zheng
• Keywords: Biomolecular Charge, Biosensor, Nanopore, Single Molecular Sensing

References

[1] J. Fritz, E. B. Cooper, S. Gaudet, P. K. Sorger, and S. R. Manalis, Electronic detection of DNA by its intrinsic molecular charge,, PNAS, vol. 99, no. 22, p.14142–14146, Oct. (2002).

DOI: 10.1073/pnas.232276699

[2] A. Poghossian, A. Cherstvy, S. Ingebrandt, A. Offenhäusser, and M. J. Schöning, Possibilities and limitations of label-free detection of DNA hybridization with field-effect-based devices,, Sensors and Actuators B: Chemical, vol. 111, p.470–480, Nov. (2005).

DOI: 10.1016/j.snb.2005.03.083

[3] N. Mojarad and M. Krishnan, Measuring the size and charge of single nanoscale objects in solution using an electrostatic fluidic trap,, Nature Nanotechnology, vol. 7, p.448–452, Jun. (2012).

DOI: 10.1038/nnano.2012.99

[4] P. Xie, Q. Xiong, Y. Fang, Q. Qing, and C. M. Lieber, Local electrical potential detection of DNA by nanowire-nanopore sensors.,, Nature Nanotechnology, vol. 7, p.119–125, Jan. (2012).

DOI: 10.1038/nnano.2011.217

[5] Graf, M.; Lihter, M.; Altus, D.; Marion, S.; Radenovic, A. Transverse Detection of DNA Using a MoS2 Nanopore. Nano Lett. 2019, 19 (12), 9075−9083.

DOI: 10.1021/acs.nanolett.9b04180

[6] Heerema, S. J.; Vicarelli, L.; Pud, S.; Schouten, R. N.; Zandbergen, H. W.; Dekker, C. Probing DNA Translocations with Inplane Current Signals in a Graphene Nanoribbon with a Nanopore. ACS Nano 2018, 12 (3), 2623−2633.

DOI: 10.1021/acsnano.7b08635

[7] M. Puster, A. Balan, J. A. Rodríguez-Manzo, G. Danda, J.-H. Ahn, W. Parkin, and M. Drndić, Cross-Talk Between Ionic and Nanoribbon Current Signals in Graphene Nanoribbon-Nanopore Sensors for Single-Molecule Detection,, Small, vol. 11, no. 47, p.6309–6316, Oct. (2015).

DOI: 10.1002/smll.201502134

[8] X. Zhu, X. Li, C. Gu, Z. Ye, Z. Cao, X. Zhang, C. Jin, and Y. Liu, Monolithic Integration of Vertical Thin-Film Transistors in Nanopores for Charge Sensing of Single Biomolecules,, ACS Nano. 2021, 15, 9882-9889.

DOI: 10.1021/acsnano.1c01042

[9] Böhme, U.; Scheler, U. Effective Charge of Bovine Serum Albumin Determined by Electrophoresis NMR. Chem. Phys. Lett. 2007, 435 (4–6), 342–345.

DOI: 10.1016/j.cplett.2006.12.068

[10] Sze, S. M.; Ng, K. Physics of Semiconductor Devices, 3rd ed.; John Wiley & Sons, Inc., Hoboken, NJ, (2006).

[11] X. Zhu, L. Guo, S. Ni, X. Zhang, and Y. Liu, Transport-Induced Inversion of Screening Ionic Charges in Nanochannels,, The Journal of Physical Chemistry Letters, p.5235–5241, Dec. (2016).

DOI: 10.1021/acs.jpclett.6b02563

[12] Y. Liu, D. E. Huber, V. Tabard-Cossa, and R. W. Dutton, Descreening of field effect in electrically gated nanopores,, Appl. Phys. Lett., vol. 97, no. 14, p.143109–4, Oct. (2010).

DOI: 10.1063/1.3497276

[13] Y. Liu, J. Sauer, and R. W. Dutton, Effect of electrodiffusion current flow on electrostatic screening in aqueous pores,, Journal of Applied Physics, vol. 103, no. 8, p.084701, (2008).

DOI: 10.1063/1.2906327