Preliminary Study of Isolation and Purification Mitragynine from Kratom Leaves

Abstract:

Kratom (Mitragyna speciosa Korth) leaves contain a major alkaloid, mitragynine, which has opioid pain-relieving activity. Kratom and its derivatives are abused as alternatives to other narcotics. Therefore, this preliminary study was aimed to optimize the extraction of mitragynine from kratom investigating the effect of extraction time and ultrasonication on yield and quality of extract (the content of mitragynine). The extraction was carried out sequential extraction at room temperature and treatment of ultrasonication before extraction which was followed the purification process. Ultrasound-assisted extraction increased mitragynine yield to16.88%, compared to extraction without ultrasonication. The extract was purified by column chromatography, eluted with a mixture of n-hexane - ethyl acetate - 25% ammonia with a ratio of 30:15:1 (v/v). Likewise, the highest mitragynine purity obtained was 86.46%.

• Periodical: Materials Science Forum (Volume 1061)
• Pages: 173-179
• DOI: https://doi.org/10.4028/p-74ggl5
• Online since: May 2022
• Authors: Neni Isnaeni*, Asep Saefumillah, Antonius Herry Cahyana
• Keywords: Isolation, Kratom, Mitragyna speciosa, Mitragynine, Purification, Ultrasound-Assisted Extraction

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