Synthesis and Characterization of Chitosan/Alginate Hydrogel Using CaCl₂ as a Crosslinking Agent

Abstract:

Hydrogels, a type of polymer, can be synthesized from both natural and synthetic sources, including some biopolymers like alginate and chitosan, making them particularly interesting for biomedical applications. The popularity of hydrogels in the medical field is due to their high-water content, flexibility, and biocompatibility. Hydrogels, which can swell in a hydrated state, are capable of controlling the release of active substances in pharmaceutical and biomedical applications. Alginate and chitosan exhibit polyanionic and polycationic properties when dissolved under appropriate conditions, allowing them to interact with each other. This interaction occurs through the carbonyl groups of alginates and the amino groups of chitosan. Alginate also has the advantages of being non-toxic, biodegradable, biocompatible, and non-allergenic. Therefore, these two materials readily form polyelectrolyte complexes. The use of calcium chloride in producing hydrogels is due to its ability to perform ionic cross-linking on polymers such as alginate. Calcium chloride reacts with the carboxylate groups in alginate, forming stable cross-links between polymer chains. This cross-linking process results in a three-dimensional network that provides structure and stability to the hydrogel. The benefits of adding CaCl₂, in addition to facilitating cross-linking, include increasing the viscosity of the alginate solution, which enhances the formation of the alginate matrix. This study demonstrates that the ratio of chitosan to alginate significantly influences the properties of the resulting hydrogel, impacting its swelling ratio, stability, and ultimately, its potential for biomedical applications. Specifically, the optimal ratio of 5A:1C exhibited superior swelling and gel fraction characteristics, suggesting its potential suitability for controlled drug delivery systems. The successful cross-linking confirmed by FTIR analysis further strengthens the viability of this specific composition for biomedical applications.

• Periodical: Materials Science Forum (Volume 1140)
• Pages: 85-91
• DOI: https://doi.org/10.4028/p-bj4cHv
• Online since: December 2024
• Authors: Azmia Rizka Nafisah*, Fadhil Muhammad Tarmidzi, Deril Setiawan, Dimas Rivaldi Ngabito
• Keywords: Alginate, Calcium Chloride, Chitosan, Hydrogel

References

[1] M. Rizwan et al., "pH Sensitive Hydrogels in Drug Delivery: Brief History, Properties, Swelling, and Release Mechanism, Material Selection and Applications," Polymers (Basel)., vol. 9, no. 4, 2017.

DOI: 10.3390/polym9040137

[2] J. Hu, Y. Chen, Y. Li, Z. Zhou, and Y. Cheng, "A thermo-degradable hydrogel with light-tunable degradation and drug release," Biomaterials, vol. 112, p.133–140, 2017.

DOI: 10.1016/j.biomaterials.2016.10.015

[3] E. Caló and V. V. Khutoryanskiy, "Biomedical applications of hydrogels: A review of patents and commercial products," Eur. Polym. J., vol. 65, p.252–267, 2015.

DOI: 10.1016/j.eurpolymj.2014.11.024

[4] S. Thakur, B. Sharma, A. Verma, J. Chaudhary, S. Tamulevicius, and V. K. Thakur, "Recent progress in sodium alginate based sustainable hydrogels for environmental applications," J. Clean. Prod., vol. 198, p.143–159, 2018.

DOI: 10.1016/j.jclepro.2018.06.259

[5] J. Wróblewska-Krepsztul, T. Rydzkowski, I. Michalska-Pożoga, and V. K. Thakur, "Biopolymers for Biomedical and Pharmaceutical Applications: Recent Advances and Overview of Alginate Electrospinning," Nanomaterials, vol. 9, no. 3, 2019.

DOI: 10.3390/nano9030404

[6] K. Baysal, A. Z. Aroguz, Z. Adiguzel, and B. M. Baysal, "Chitosan/alginate crosslinked hydrogels: Preparation, characterization and application for cell growth purposes," Int. J. Biol. Macromol., vol. 59, p.342–348, 2013.

DOI: 10.1016/j.ijbiomac.2013.04.073

[7] S. Houben and L. M. Pitet, "Ionic crosslinking strategies for poly(acrylamide) /alginate hybrid hydrogels," React. Funct. Polym., vol. 191, p.105676, 2023, doi: https://doi.org/10.1016/ j.reactfunctpolym.2023.105676.

DOI: 10.1016/j.reactfunctpolym.2023.105676

[8] I. Kolesnyk, V. Konovalova, and A. Burban, "Alginate/κ-Carrageenan Microspheres and their Application for Protein Drugs Controlled Release," Chem. ans Chem. Technol., vol. 9, pp.485-492, 2015.

DOI: 10.23939/chcht09.04.485

[9] N. F. A.-Z. Tuan Mohamood, A. H. Abdul Halim, and N. Zainuddin, "Carboxymethyl Cellulose Hydrogel from Biomass Waste of Oil Palm Empty Fruit Bunch Using Calcium Chloride as Crosslinking Agent," Polymers (Basel)., vol. 13, no. 23, 2021.

DOI: 10.3390/polym13234056

[10] A. Prasetyaningrum et al., "Encapsulation of Lemongrass Extract (Cymbopogon citratus) Coated Alginate/Chitosan Using Gelation Method," Reaktor, vol. 21, no. 3, p.124–132, 2021.

DOI: 10.14710/reaktor.21.3.124-132

[11] R. Abka-Khajouei, L. Tounsi, N. Shahabi, A. K. Patel, S. Abdelkafi, and P. Michaud, "Structures, Properties and Applications of Alginates.," Mar. Drugs, vol. 20, no. 6, May 2022.

DOI: 10.3390/md20060364

[12] K. Baysal, A. Z. Aroguz, Z. Adiguzel, and B. M. Baysal, "Chitosan/alginate crosslinked hydrogels: Preparation, characterization and application for cell growth purposes," Int. J. Biol. Macromol., vol. 59, no. May, p.342–348, 2013.

DOI: 10.1016/j.ijbiomac.2013.04.073

[13] D. R. Barleany et al., "Hydrogel Preparation from Shrimp Shell-Based Chitosan: The Degree of Crosslinking and Swelling Study," ASEAN J. Chem. Eng., vol. 23, no. 1, p.28–39, 2023.

DOI: 10.22146/ajche.73716

[14] M. T. ALSamman and J. Sánchez, "Chitosan- and Alginate-Based Hydrogels for the Adsorption of Anionic and Cationic Dyes from Water," Polymers (Basel)., vol. 14, no. 8, 2022.

DOI: 10.3390/polym14081498

[15] M. T. ALSamman and J. Sánchez, "Recent advances on hydrogels based on chitosan and alginate for the adsorption of dyes and metal ions from water," Arab. J. Chem., vol. 14, no. 12, p.103455, 2021.

DOI: 10.1016/j.arabjc.2021.103455

[16] D. Tahtat, M. Mahlous, S. Benamer, A. N. Khodja, H. Oussedik-Oumehdi, and F. Laraba-Djebari, "Oral delivery of insulin from alginate/chitosan crosslinked by glutaraldehyde," Int. J. Biol. Macromol., vol. 58, p.160–168, 2013.

DOI: 10.1016/j.ijbiomac.2013.03.064