The Influence of Different Initial Relative Densities and Ambient Temperature Ranges on MICP Treatment Using Direct-Mixing Injection Method

Article Preview

Abstract:

Microbially induced calcite precipitation (MICP) is a promising alternative method for improving the geotechnical properties of granular soils. The effectiveness of the MICP technique depends on several variables, including relative density and temperature. The objective of this study was to determine the effect of different initial relative densities and ambient temperature ranges on the effectiveness of MICP. After 300 and 600 hours of MICP treatment, with injection cycles occurring every 12 hours, the specimens with a relative density of 34.5% were found to be effectively cemented. In contrast, specimens with a relative density of 59.8% were found to be less cemented. A greater percentage of specimens (52.4%) were cemented at warmer ambient temperatures (20-28°C), compared to only 15.7% at cooler temperatures (16-24°C). These results suggest that the looser soil matrix and warmer temperatures facilitated CaCO3 precipitation and resulted in greater cementation.

You might also be interested in these eBooks

Info:

Periodical:

Materials Science Forum (Volume 1178)

Pages:

133-141

Citation:

Online since:

February 2026

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2026 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] P. Xiao, H. Liu, A.W. Stuedlein, T.M. Evans, Y. Xiao, Effect of relative density and biocementation on cyclic response of calcareous sand, Canadian Geotechnical Journal. 56 (12) (2019) 1849–1862

DOI: 10.1139/cgj-2018-0573

Google Scholar

[2] J. Chu, V.Ivanov, J. He, M. Naemi, B. Li, V. Stabnikov, Development of microbial geotechnology in Singapore, Geofrontiers. (2011) 4070–4078

DOI: 10.1061/41165(397)416

Google Scholar

[3] J.T. DeJong, M.B. Fritzges, K. Nusslein, Microbially induced cementation to control sand response to undrained shear, J. Geotech. Geoenviron. 132 (11) (2006) 1381−1392

DOI: 10.1061/(ASCE)1090-0241(2006)132:11(1381)

Google Scholar

[4] F. Chen, C. Deng, W. Song, D. Zhang, F.A. Al-Misned, M.G. Mortuza, G.M. Gadd, X. Pan, Biostabilization of desert sands using bacterially induced calcite precipitation, Geomicrobiology Journal. 33 (3-4) (2016) 243–249

DOI: 10.1080/01490451.2015.1053584

Google Scholar

[5] F. Pacheco-torgal, V. Ivanov, N. Karak, H. Jonkers, Biopolymers and biotech admixtures for eco-efficient construction materials, Woodhead Publishing. (2016)

DOI: 10.1016/B978-0-08-100214-8.01001-0

Google Scholar

[6] L. Cheng, M.A. Shahin, R. Cord-Ruwisch, M. Addis, T. Haranto, C. Elms, Soil stabilisation by microbial induced calcium carbonate precipitation: investigation of some important physical and environmental aspects, Proceedings of the 7th International Congress on Environmental Geotechnics, 10 - 14 November, Melbourne, Australia.

Google Scholar

[7] R. Siddique, N. Chahal, Effect of ureolytic bacteria on concrete properties, Constr. Build. Mater. 25 (10) (2011) 3791–3801

DOI: 10.1016/j.conbuildmat.2011.04.010

Google Scholar

[8] S. Khosthtinat, Advancements in exploiting Sporosarcina pasteurii as sustainable construction material: a review, Sustainability. 15 (18) (2023) 13869

DOI: 10.3390/su151813869

Google Scholar

[9] K.L. Sahrawat, Effects of temperature and moisture on urease activity in semi-arid tropical soils, Plant Soil. 78 (1984) 401–408

DOI: 10.1007/BF02450373

Google Scholar

[10] L.A. Van Paassen, Biogrout: Ground Improvement by Microbial Induced Carbonate Precipitation, Delft University of Technology, Delft, 2009.

Google Scholar

[11] F.J. Kadhim, J. Zheng, Review of the factors that influence on the microbial induced calcite precipitation, Civil and Environmental Research. 8 (10) (2016) 69–76.

Google Scholar

[12] BMKG, Anomali Suhu Udara Rata-Rata Bulan September 2023, Badan Meteorologi, Klimatologi, dan Geofisika, Jakarta, 2024. https://www.bmkg.go.id/iklim/?p=ekstrem-perubahan-iklim

DOI: 10.31172/jmg.v16i1.260

Google Scholar

[13] NREL, Geothermal Heat Pump Basics, National Renewable Energy Laboratory, Colorado, 2024. https://www.nrel.gov/research/re-geo-heat-pumps.html

DOI: 10.2172/878494

Google Scholar

[14] M. Li, K. Wen, Y. Li, L. Zhu, Impact of oxygen availability on microbially induced calcite precipitation (MICP) treatment, Geomicrobiol. J. 35 (1) (2017) 15–22

DOI: 10.1080/01490451.2017.1303553

Google Scholar

[15] G. Le Metayer-Levrel, S. Castanier, G. Orial, J. Loubière, J. Perthuisot, Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony, Sediment. Geol. 126 (1999) 25–34

DOI: 10.1016/S0037-0738(99)00029-9

Google Scholar

[16] R.A. Sudisman, I.M. Ningrum, S. Sastroredjo, A.A. Cikmit, A.A. Zulfah, S.D.T. Wewengkang, Evaluating bio-cementation injection techniques for silica sand: duration, distribution, and strength enhancement, GEOMATE J. 28 (129) (2025) 55–65. https://geomatejournal.com/geomate/article/view/4855

DOI: 10.21660/2025.129.4855

Google Scholar

[17] S. Stocks-Fisher, J. Galinat, S. Bang, Microbiological precipitation of CaCO3, Soil Biol. Biochem. 31 (11) (1999) 1563–1571

DOI: 10.1016/S0038-0717(99)00082-6

Google Scholar

[18] G. Kim, J. Kim, H. Youn, Effect of temperature, pH, and reaction duration on microbially induced calcite precipitation, Appl. Sci. 8 (8) (2018) 1277

DOI: 10.3390/app8081277

Google Scholar