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The Impact of Silver Nanoparticles on Small Intestine Cells and Oocytes Follicular Environment Cells in Female Mice
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The Impact of Silver Nanoparticles on Small Intestine Cells and Oocytes Follicular Environment Cells in Female Mice

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Abstract:

The impact of silver nanoparticles (AgNPs) on the intestine and reproductive system have not been sufficiently studied. Meanwhile, the relationship between disruption of the intestinal epithelial barrier and female reproductive disorders is under active investigation.The aim of the work was to evaluate the effects of AgNPs on small intestine cells and oocytes follicular environment cells under conditions of experimental premature ovarian failure (ePOF) and the use of Resveratrol in female mice.Methods. ePOF was modeled by immunizing with a suspension of kidney antigen (10 μl per 10 g of weight) according to the scheme. AgNPs (2,0 mg/kg) and Resveratrol (RES, 50,0 mg/kg) was administered intraperitoneally once a day three times after the last immunization.Enterocytes were isolated and cell death was assessed by the method of two-color fluorescent nucleic acid dye. Analyzed data with GraphPad Prism.Results. It was found that AgNPs under ePOF conditions did not cause significant changes in the viability of ileal enterocytes. In cells of the follicular environment of oocytes, under the influence of nanoparticles, a 1.40-fold decrease in the proportion of living cells and an increase in the proportion of cells with morphological signs of apoptosis and necrosis were observed, by 1.17 times and 1.43 times, respectively (p<0.05, n=6).Under ePOF conditions, the use of RES after AgNPs caused a 1.15-fold increase in the proportion of live cells in the ileum and a 1.43-fold decrease in apoptotic cells. In the follicular environment of oocytes, the proportion of live cells increased 1.54 times, and the proportion of cells with morphological signs of apoptosis and necrosis decreased 1.28 and 1.62 times, respectively (p<0.05, n=6).Conclusions. New data on the impact of AgNPs on small intestinal cells and the oocytes follicular environment cells have been obtained, encouraging further research possible therapeutic treatment of such nanoparticles.

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Periodical:

Materials Science Forum (Volume 1179)

Pages:

13-18

DOI:

https://doi.org/10.4028/p-Fv0NNL

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Online since:

March 2026

Authors:

Alina Lytvynenko*, Orest Blashkiv, Valentyna Sribna

Keywords:

Branched Copolymers, Enterocytes, Experimental Premature Ovarian Failure, Oocytes Follicular Environment Cells, Regulated Cell Death, Resveratrol, Silver Nanoparticles

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© 2026 Trans Tech Publications Ltd. All Rights Reserved

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