A constitutive equation has been established to describe the effect of grain size on the deformation behavior of Ti-6.62Al-5.14Sn-1.82Zr alloy during the high temperature. In this paper, firstly a steady flow stress model is proposed, and a function relating to the grain size is introduced to modify the steady flow stress model. Meanwhile, a microstructure model established by the fuzzy neural network method is applied to calculate the grain size of prior α phase during the high temperature deformation of Ti-6.62Al-5.14Sn-1.82Zr alloy. The calculated flow stress using the present constitutive equation shows a good agreement with the experimental flow stress of the Ti-6.62Al-5.14Sn-1.82Zr alloy. The relative maximum error was not more than 15%.