Inhibitory Effect of Hydroxyapatite Nanoparticles on K562 Cells

Abstract:

HAP Nanoparticles Was Synthesized by Homogeneous Precipitation. the Size Distribution, Crystallization Degree and Morphology of the Precipitation Were Characterized by Laser Granularity Instrument, X-Ray Diffraction (XRD), and Transmission Electron Microscope (TEM) Respectively. the Prepared HAP Nanoparticles Were Used for the Treatment of Human Chronic Myeloid Leukemia K562 Cells. the Inhibition Effect of the Nanoparticles on the Proliferation of K562 Cells Was Measured by MTT Assay and Growth Curve Test. the Results Showed that the HAP Nanoparticles Inhibit the Proliferation of K562 Cells Dramatically in Vitro. the Likely Inhibition Mechanism of HAP Nanoparticles on the K562 Cells Is that the Nanoparticles Entered into the Dells, Induced a Series of Cell Changes, through Cell Death of Apoptosis, Oncosis and Autoschizis, Thus Led to the Death of K562 Cells.

• Periodical: Materials Science Forum (Volume 685)
• Pages: 352-356
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.685.352
• Online since: June 2011
• Authors: Hong Lian Dai, Pei Chen, Yin Chao Han, Xin Yu Wang, Shi Pu Li
• Keywords: Cell Cycle, Hydroxyapatite （HAP） Nanoparticles, K562 Cells, MTT Assay

References

[1] L.Y. Feng, S.P. Li, Y.H. Yan, et al. Chinese J of Biomedical Engineering (2001), pp.302-306.

[2] X.Y. Cao, Z.T. Qi, H.L. Dai, et al. Joural of Wuhan University of Technology-Mater. Sci. Ed, Vol. 18(3), (2003), pp.66-68.

[3] Y.F. Wang, Y.H. Yan, W. Ren, et al. Chain, Vol. 14(1), (2004), pp.29-32.

[4] S. Hu, Y.H. Yan, Y.F. Wang, et al. Chinese Journal of Biomedical Engineering, Vol. 24(4), (2005), pp.482-485.

[5] G. Majno, I. Joris. Am. J. Pathol., Vol. 146, (1995), pp.3-15.

[6] M.J. Jamison, J. Gilloteaux, H.S. Taper, et al. Biochem. Pharm., Vol. 63, (2002), pp.1773-1783.