Antibacterial Activity of Ag-TiO₂ Nanoparticles with Various Silver Contents

Abstract:

Bacterial are highly transmitted in our environment and have been identified as a primary contributor to the problem of indoor air quality and consequently lead to the illness of the occupants. Recently, nanotechnology represents an innovative approach to develop new formulations based on metallic nanoparticles with antimicrobial properties. TiO₂ has great promise to diminish bacterial activity. Antimicrobial activity of TiO₂and Ag-TiO₂ nanoparticles against Escherichia coli was examined in this study. TiO₂ nanoparticles with various silver contents were synthesized by sol gel method to produce uniform size, unagglomerated state and homogeneous nanoparticles. The nanoparticles were characterized by X-Ray diffraction (XRD) and transmittance electron microscopy (TEM). The effects of different silver concentration were studied using cotton diffusion test under fluorescence light irradiation. 0.06 mol % Ag-TiO₂ revealed best antibacterial activity. 0.06 mol % Ag-TiO₂ have antibacterial inhibition zone of 38 mm at the concentration of 2.0 M against E. coli. Swab test bacterial counts on left palm, tile, mouse pad and cotton have been tested before and after spraying with 0.06 mol % Ag-TiO₂. It showed that the bacterial count decreased for entire samples. The significant enhancement in the antibacterial properties of Ag-TiO₂ nanoparticles under visiblelight irradiation is related to the effect of noble metal Ag by acting as electron traps in TiO₂ band gap. The phase structure, crystallite size and crystallinity of TiO₂ also play an important role inantibacterial activity. The killing mechanism of Ag-TiO₂ undervisible light irradiation antibacterial activity over Ag-TiO₂nanoparticles was proposed based on our observations.

• Periodical: Materials Science Forum (Volume 756)
• Pages: 238-245
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.756.238
• Online since: May 2013
• Authors: Nur Hidayati Ahmad Barudin, Srimala Sreekantan, Ong Ming Thong, Geethaa Sahgal
• Keywords: Escherichia coli, Silver, Sol Gel, TiO₂ Nanoparticles

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