Biosynthesis Silver Nanoparticles Using Bacillus Amyloliquefaciens Zxw01 and Research on Synthesis Mechanism

Abstract:

This research reported on synthesis of silver nanoparticles using Bacillus amyloliquefaciens zxw01 culture mixed with silver nitrate. The nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD), Transmission electron microscopy (TEM) and high-resolution transmission electron microscope (HRTEM).In addition, we discussed synthesis mechanism by comparing the protein files of the bacteria before and after mixed with silver nitrate and proteins attached to silver nanoparticles. Our results indicated that silver nanoparticles biosynthesized by Bacillus amyloliquefaciens zxw01 were equally distributed with size between 5 nm to 30 nm and face-centred cubic structure; results of SDS-PAGE suggested that after mixed with silver nitrate, the bacteria differentially expressed and produced a new protein with weight of 33 kDa. Furthermore, analysis of proteins attached to silver nanoparticles indicated that protein with weight of 33 kDa was related to the synthesis of silver nanoparticles.

• Periodical: Materials Science Forum (Volume 852)
• Pages: 437-442
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.852.437
• Online since: April 2016
• Authors: Jie Zhang, Ying Zhang, Bin Song Wang, Maria Shishova
• Keywords: Bacillus Amyloliquefaciens, Bioreduction, Reducing Mechanism, SDS-PAGE, Silver Nanoparticles

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