Preparation and Blood Coagulation Property of Ortho Hydroxytoluene Chitosan

Abstract:

The aim of this paper was to synthesize ortho hydroxytoluene chitosan for hemostatic use. A series of modified chitosan were prepared by reductive amination through adjusting the dosages of salicylaldehyde and sodium borohydride. FTIR, elemental analysis and XRD tests were performed to analyze the composition, degree of substitution (DS) and crystallisability of chitosan derivatives. Rheological and blood coagulation experiments were carried out to measure its gel-forming and hemostasis property in vitro. The results showed ortho hydroxytoluene groups could be grafted on chitosan, DS value can be adjusted by the dosage of reactive agents, and the crystallinity of chitosan changed obviously after modification. The rheological results indicated high substituted (≥26.79 mol-%) ortho hydroxytoluene chitosan could gel blood and showed better blood coagulation property. High substituted ortho hydroxytoluene chitosan could improve hemostatic property significantly.

• Periodical: Materials Science Forum (Volume 893)
• Pages: 62-68
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.893.62
• Online since: March 2017
• Authors: Jing Guan*, Qin He, Xiao Hui Wang, Shu Jie Huang, Zhi Hong Li, Chang Jun Liu, Fan Li, Zhan Shu
• Keywords: Blood Coagulation, Crystallinity, Degree of Substitution, Ortho Hydroxytoluene Chitosan, Rheological Behaviour

References

[1] J. G. Chapman, N. Jacobs, M.J. Midwinter, Pre-hospital haemostatic dressings: A systematic review, Injury, Int. J. Care Injured 42 (2011), 447-459.

DOI: 10.1016/j.injury.2010.09.037

[2] J.F. Kelly, A.E. Ritenour, D.F. McLaughlin, Injury severity and causes of death from Operation Iraqi Freedom and Operation Enduring Freedom: 2003–2004 versus 2006, J. Trauma 64 (2008), 21–27.

DOI: 10.1097/ta.0b013e318160b9fb

[3] M.A. Brown, M.R. Daya, J.A. Worley, Esperience with chitosan dressings in a cilvilian EMS system, J Emerg. Med. 37 (2009), 1–7.

[4] B. Gegel, J. Burgert, B. Cooley, J. MacGregor, J. Myers, S. Calder, R. Luellen, M. Loughren, D. Johnson, The effects of BleedArrest, Celox, and TraumaDex on hemorrhage control in a porcine model, J. Surg. Res. 164 (2010), e125–e129.

DOI: 10.1016/j.jss.2010.07.060

[5] W.A. Sakchai, P. Chureerat, Development and in vitro evaluation of chitosan–polysaccharides composite wound dressings, Int. J. Pharm. 313 (2006), 123-128.

[6] X.F. Huang, Y.F. Sun, J.Y. Nie, W.T. Lu, L. Yang, Z.L. Zhang, H.P. Yin, Z.K. Wang, Q.L. Hu, Using absorbable chitosan hemostatic sponges as a promising surgical dressing, Int. J Biol. Macromol. 75 (2015), 322–329.

DOI: 10.1016/j.ijbiomac.2015.01.049

[7] B.K. Gu, S.J. Park, M.S. Kim, C.M. Kang, J. Kim, C.H. Kim, Fabrication of sonicated chitosan nanofiber mat with enlarged porosity for use as hemostatic materials, Carbohydr. Polym. 97 (2013), 65-71.

DOI: 10.1016/j.carbpol.2013.04.060

[8] M.B. Dowling, R Kumar, M.A. Keibler, J.R. Hess, G.V. Bochicchio, S.R. Raghavan, A self-assembling hydrophobically modified chitosan capable of reversible hemostatic action, Biomaterials 32 (2011), 3351-3357.

DOI: 10.1016/j.biomaterials.2010.12.033

[9] R. Muzzarelli, Removal of uranium from solutions and brines by a derivative of chitosan and ascorbic acid, Carbohydr. Polym. 5(1985), 85-89.

DOI: 10.1016/0144-8617(85)90026-8

[10] M. Rinaudo, New Amphiphilic Grafted copolymers based on polysaccharides, Carbohydr. Polym. 83 (2011), 1338-1344.

DOI: 10.1016/j.carbpol.2010.09.054

[11] Z.M. dos Santos, A.L.P.F. Caroni, M.R. Pereira, D. R. da Silva, J.L.C. Fonseca, Determination of Deacetylation Degree of Chitosan: A Comparison between Conductometric Titration and CHN Elemental Analysis, Carbohydr. Res. 344 (2009), 2591- 2595.

DOI: 10.1016/j.carres.2009.08.030

[12] O. Ornella, D. Gerardino, M. Gaetano, C. Donato, The aggregative behavior of hydrophobically modified chitosans with high substitution degree in aqueous solution, Carbohydr. Polym. 74 (2008), 16–22.

DOI: 10.1016/j.carbpol.2008.01.009

[13] J. Desbrières, C. Martinez, M. Rinaudo, Hydrophobic derivatives of chitosan: characterization and rheological behavior, Int. J. Biol. Macromol. 19 (1996), 21-28.

DOI: 10.1016/0141-8130(96)01095-1

[14] G.P. Ma, D.Z. Yang, Y.S. Zhou, M. Xiao, J.F. Kennedy, J. Nie, Preparation and characterization of water-soluble N-alkylated chitosan, Carbohydr. Polym. 74 (2008), 121-126.

DOI: 10.1016/j.carbpol.2008.01.028

[15] Y.Y. Li, S.S. Zhang, X.J. Meng, X.G. Chen, G.D. Ren, The Preparation and characterization of a novel amphiphilic oleoyl-carboxymethyl chitosan self-assembled nanoparticles, Carbohydr Polym. (83) 2011, 130-136.

DOI: 10.1016/j.carbpol.2010.07.030

[16] K. Almdal, J. Dyre, S. Hvidt, O. Kramer, Towards a phenomenological definition of the term gel, Polym. Gels Networks 1 (1993), 5-17.

DOI: 10.1016/0966-7822(93)90020-i

[17] Q. He, J. Guan, M.L. Jing, S.J. Huang, C. Xu, Z.H. Li, J.M. Wu, X.Z. Zhang, Synthesis and characterization of hydrophobically modified chitosan, Afr. J Pharm. Pharmaco. 6 (2012), 3285-3292.

DOI: 10.5897/ajpp12.1427