Antimicrobial Activity of Chlorhexidine against Multi-Species Biofilm Formation

Abstract:

In the present work, the efficacy of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine against bacterial biofilm formation was evaluated. Nanotubes were processed using anodization in 0.25% NH₄F electrolyte solution. Biofilms were cultured in discs immersed in sterile brain heart infusion broth (BHI) containing 5% sucrose, inoculated with microbial suspension (106 cells/ml) and incubated for 5 days. Next, the discs were placed in tubes with sterile physiological solution 0.9% sodium chloride (NaCl) and sonicated to disperse the biofilms. Tenfold serial dilutions were carried and aliquots seeded in selective agar, which were then incubated for 48 h. Then, the numbers CFU/ml (log 10) were counted and analyzed statistically. Scanning electron microscopy (SEM) on discs with biofilms groups and contact angle was carried out. The results show that there is no difference in bacterial adhesion between of the Ti–7.5Mo alloy nanotube and Ti–7.5Mo alloy nanotube with chlorhexidine.

• Periodical: Materials Science Forum (Volume 899)
• Pages: 237-242
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.899.237
• Online since: July 2017
• Authors: Ana Lucia do Amaral Escada, Cristiane Aparecida Pereira, Antonio Olavo Cardoso Jorge, Ana Paula Rosifini Alves Claro
• Keywords: Biofilm, Chlorhexidine, Nanotube TiO₂, Titanium Alloy

References

[1] W. Heuer, C. Elter, A. Demling, A. Neumann, S. Suerbaum, M. Hannig: Journal of Oral Rehabilitation Vol. 34 (2007), p.377.

DOI: 10.1111/j.1365-2842.2007.01725.x

[2] M.M. Fürst, G.E. Salvi, N.P. Lang, G.R. Persson: Clinical Oral Implants Research Vol. 18 (2007), p.501.

[3] J. Li, E.J. Helmerhorst, C.W. Leone, R.F. Troxler, T. Yaskell, A.D. Haffajee: Journal of Applied Microbiology Vol. 97 (2004), p.1311.

DOI: 10.1111/j.1365-2672.2004.02420.x

[4] H.J. Busscher, R.J. Ploeg H.C. Van der Mei: Biomaterials Vol. 30 (2009), p.4247.

[5] T. Kuyyakanond, L.B. Quesnel: FEMS Microbiol. Lett. Vol. 15 (1992), p.211.

[6] S. Schou, T. Berglundh, N.P. Lang: Int. J. Oral Maxillofac. Implants Vol. 19 (2004), p.140.

[7] P.M. Trejo, G. Bonaventura, D. Weng, R. Caffesse, U. Bragger, N.P. Lang: Clin. Oral Implant Res. Vol. 17 (2006), p.294.

DOI: 10.1111/j.1600-0501.2005.01226.x

[8] A. Kozlovsky, Z. Artzi, O. Moses, N. Kamin-Belsky, R.B. Greenstein: J. Periodontol. Vol. 77 (2006), p.1194.

[9] M.P.J. Young, M. Korachi, D.H. Carter, H.V. Worthington, J.F. McCord, D.B. Drucker: Clin. Oral Implant Res. Vol. 13 (2002), p.20.

[10] C.A. Pereira, R.L. Romeiro, A.C. Costa, A.K. Machado, J.C. Junqueira, A.O. Jorge: Lasers Med. Sci. Vol. 26 (2011), p.341.

[11] A.M. Roos-Jansaker, C. Lindah, H. Renvert, S. Renvert: Journal of Clinical Periodontology Vol. 33 (2006), p.290.

DOI: 10.1111/j.1600-051x.2006.00906.x

[12] D. Campoccia, L. Montanaro, C. Arciola: Biomaterials Vol. 27 (2006), p.2331.

[13] A. Pier-Francesco, R.J. Adams, M.G. Waters, D.W. Williams: Clinical Oral Implants Research Vol. 17 (2006), p.633.

[14] P.S. Stewart, J.W. Costerton: Lancet Vol. 358 (2001), p.135.

[15] P.C. Bonez, C.F.S. Alves, T.V. Dalmolin, V.A. Agertt, C.R. Mizdal, V.C. Flores, J.B. Marques, R.C.V. Santos, M.M. A Campos: American Journal of Infection Control Vol. 41 (2013), p.119.

DOI: 10.1016/j.ajic.2013.05.002

[16] H. Lamfon, S.R. Porter, M. McCullough, J. Pratten: J. Antimicrob. Chemother Vol. 53 (2004), p.383.

[17] K. Totè, T. Horemans, D. Vanden Berghe, L. Maes, P. Cos: Appl Environ Microbiol Vol. 76 (2010), p.3135.

[18] F.B. Zanatta, C.K. Rosing: Sci. A Vol. 1 (2007), p.35.